短期UVB辐照对抱茎风毛菊光合作用的影响  

The Influence of Short-term UVB Irradiation on Saussurea Chingiana Photosynthesis

作　　者：常宗强[1] 祁淑云[2] 

机构地区：[1]中国科学院寒区旱区环境与工程研究所,甘肃兰州730000 [2]中国农业科学院兰州兽医研究所,甘肃兰州730046

出　　处：《甘肃科学学报》2016年第4期28-33,共6页Journal of Gansu Sciences

基　　金：国家自然科学基金项目(31370396);甘肃省自然科学基金项目(145RJZA052)

摘　　要：以抱茎风毛菊(Saussurea chingiana)为实验材料,利用短期中波紫外线(UVB,Ultraviolet B)辐照对比实验(CK:对照组,自然大气UVB强度;UVB:UVB辐照度为205μw·cm^(-2),短期辐照时间为4h)研究了光合色素含量、叶绿素荧光快速诱导动力学曲线(OJIP)和光合蛋白表达量的变化。结果显示:UVB辐照降低了抱茎风毛菊的叶绿素质量分数,增大了叶绿素a/b比值和类胡萝卜素质量分数;UVB辐照导致PSII反应中心蛋白(D1、D2)、捕光色素蛋白(CP43)、Cytb6f复合物和ATP酶复合体蛋白均发生降解,外周捕光色素蛋白(LHCII)的表达量升高;UVB辐照抑制了从QA-往下的电子传递,增加了热耗散能量,阻碍了光合作用的碳同化过程,进而降低光能利用率。叶绿素a/b比值、类胡萝卜素含量的增大虽然缓解了短期UVB辐照对抱茎风毛菊的伤害,但是UVB辐照对光合作用仍具有显著的负影响。Takes Saussurea chingiana as the experimental material,and the it uses short-term wave Ultraviolet B（UVB）irradiation as the comparative experiments（CK：control group,natural atmosphere UVB intensity;UVB：UVB irradiance of 205μw·cm-2,short-term irradiation time of 4h）,which has studied the changes of photosynthetic pigment content,chlorophyll fluorescence rapid induction kinetics curves（OJIP）and photosynthetic protein expression.The results showes that：UVB irradiation reduces the chlorophyll mass fraction of Saussurea chingiana,and increases the chlorophyll a/b ratio and carotenoid mass fraction;UVB irradiation has led the degradation of PSII reaction center protein（D1,D2）,light-harvesting chlorophyll protein（CP43）,Cytb6 fcompound and ATP enzyme compound protein,and the increase of expression quantity of the light harvesting pigment-protein（LHCII）.UVB irradiation inhibits the downward electron transfer of QA-,increasing the heat dissipation energy,hindering the carbon assimilation process of photosynthesis,thus reducing the efficiency for solar energy utilization.Although the increase of chlorophyll a/b ratio and carotenoid content has eased the damage of UVB irradiation tp the Saussurea chingiana,UVB irradiation still has a significant negative impact on photosynthesis.

关 键 词：叶绿素荧光 光合色素 光合蛋白 抱茎风毛菊 UVB 

分 类 号：Q945[生物学—植物学]