超声介导载紫杉醇微泡对小鼠H22皮下移植瘤抑制作用的实验研究  

Inhibitory Effects of Ultrasound-mediated Paclitaxel-loaded Microbubbles on H22 Transplantation Tumor in Mice

作　　者：于姣姣[1] 赵云[1] 刘朝奇[1] 胡兵[2] 黄晶晶[1] 赵苗[1] 

机构地区：[1]三峡大学医学院,肿瘤微环境与免疫治疗湖北省重点实验室,湖北省宜昌市443000 [2]三峡大学第二临床医学院超声科

出　　处：《中国超声医学杂志》2016年第9期833-836,共4页Chinese Journal of Ultrasound in Medicine

摘　　要：目的探讨超声介导自制载紫杉醇微泡对小鼠H22皮下移植瘤的抑制效果。方法 (1)自制载紫杉醇微泡,并检测其一般特性。(2)建立小鼠H22皮下移植瘤模型,给予紫杉醇微泡+超声处理。绘制肿瘤生长曲线,计算各组抑瘤率,肿瘤行病理组织学检查,免疫组织化学法检测微血管密度(MVD),Western Blotting检测Bax、Bcl-2蛋白的表达。结果 (1)微泡呈圆形,表面光滑,平均粒径2.07μm,载药量10.13%,包封率89.58%。(2)紫杉醇微泡+超声组肿瘤生长明显减慢,肿瘤体积和质量明显下降(P<0.05),肿瘤组织HE染色呈现不同程度变性坏死,MVD表达减少,Bax的表达上调,Bcl-2的表达下调(P<0.05)。结论超声介导载紫杉醇微泡可明显抑制小鼠H22皮下移植瘤的生长,并可降低肿瘤组织MVD的表达,促进肿瘤细胞的凋亡。Objective To evaluate the Inhibitory effects of Ultrasound-mediated paclitaxel-loaded microbubbles on H22 transplantation tumor in mice. Methods （1） The paclitaxel-loaded microbubbles＇ properties were studied contained morphology, size distribution, concentration, drug entrapment efficiency and drug-loading amount. （2） We established H22 transplantation tumor models in mice, then mice were treated with paclitaxet-loaded microbubbles plus ultrasound, the tumor growth curve was drew according to the tumor volume. The rate of tumor inhibition was calculated after sacrificing the animals at the end of experiment, tumor tissues were examined on histopathology, mierovessel density（MVD） were measured immunohistochemically, protein expression of Bcl-2 and Bax was detected by Western Blotting. Results （1） The microbubbles were round and uniform with the mean size of 2.07 μm, The drug loading and encapsulation efficiency of paclitaxel-loaded microbubbles were 10.13 % and 89.58%. （2） Compared with control group, the tumors of paclitaxel-loaded microbubbles plus ultrasound group grew the most slowly, tumor volume and weight were significantly decreased, Paclitaxel-loaded microbubbles plus ultrasound group showed varying degrees of degeneration and necrosis in HE staining, MVD and Bcl-2 expression were decreased, Bax expression was increased （P〈0.05）. Conclusions Ultrasound-mediated paclitaxel-loaded microbubbles can inhibit the growth of H22 trans-plantation tumor in mice, reduce the expression of MVD and promote apoptosis of the tumor cells.

关 键 词：超声 载紫杉醇微泡 H22皮下移植瘤 

分 类 号：R965[医药卫生—药理学]