微生物合成黄酮糖苷类天然产物研究进展  

Advances on Flavonoid Glycosides Production of Engineered Microorganisms

作　　者：李晓波[1] 刘雪[1] 赵广荣[1] 

机构地区：[1]天津大学化工学院制药工程系系统生物工程教育部重点实验室天津化学化工协同创新中心合成生物学平台,天津300072

出　　处：《中国生物工程杂志》2016年第8期105-112,共8页China Biotechnology

基　　金：国家"863"计划(2012AA02A701);国家重点基础研究发展计划(2011CBA00800);国家自然科学基金(31570087);天津市应用基础与前沿技术研究计划(13JCZDJC27600)资助项目

摘　　要：黄酮糖苷类天然产物是植物中黄酮类化合物的主要存在形式,通过糖基化修饰,可以改变其水溶性、稳定性等,赋予其新的生物活性和功能。黄酮类化合物的糖基化修饰通常由植物源或微生物源的糖基转移酶催化,根据糖基的位置、类型和数量的不同,可形成多种类型的黄酮糖苷类产物。随着合成生物学和代谢工程的快速发展,在微生物中合成植物源黄酮糖苷类天然产物取得了重要进展。综述了糖基转移酶的聚类分析及糖基供体的途径改造,并对代谢工程优化黄酮糖苷类天然产物的微生物合成进行了分析讨论,并对其发展前景进行了展望。Flavonoid glycosides are a large group of plant-based phenolic nature products. The glycosylation of flavonoids increases their solubility and stability relative to flavonoid aglycones, mainly catalyzed by glycosyltransferases （Gts）. Uridine diphosphate glycosyhransferases play a key role in decorating flavonoids with different sugars, resulting in numerous structurally diverse flavonoid glycosides. With the rapid development of synthetic biology and metabolic engineering, engineered yeast, Escherichia coli and other microorganisms have been utilized to construct the biosynthesis pathways of sugar donors and flavonoid aglycones. The recent progress on glycosylated flavonoids in engineered microorganisms, as well as the clustering of glycosyltransferase, biosynthetic pathway of sugar donors were summarized, and its future trend was explored.

关 键 词：黄酮糖苷类 合成生物学 代谢工程 工程微生物 

分 类 号：Q819[生物学—生物工程]