缬芎滴丸对大鼠脑缺血再灌注损伤后血管新生的影响  

Effect of Valerian Ligusticum Pill on angiogenesis after injury of cerebral ischemia reperfusion in rats

作　　者：干良敏 魏守蓉[1] 向本旭 王文[2] 薛存宽[1] 

机构地区：[1]华中科技大学同济医学院附属梨园医院,湖北武汉430077 [2]首都医科大学宣武医院,北京100053

出　　处：《药学学报》2016年第9期1423-1428,共6页Acta Pharmaceutica Sinica

基　　金：湖北省科技厅自然科学基金资助项目(2014CBF957)

摘　　要：探讨中药复方缬芎滴丸对大鼠脑缺血再灌注损伤的作用及其在血管新生方面的机制。60只SD大鼠随机分为假手术组、模型组、缬芎滴丸低剂量(30 mg·kg-1)组、缬芎滴丸高剂量(50 mg·kg^(-1))组和尼莫地平(10mg·kg^(-1))组,线栓法制作大鼠大脑中动脉闭塞(MCAO)模型,缺血30 min再灌注,假手术组大鼠只结扎颈总动脉,不插栓。大鼠清醒后进行第1次神经功能评分,然后对缬芎滴丸低、高剂量组和尼莫地平组大鼠灌胃给药,每次2 m L/只,每天1次,共7天,每次均在同一时间点,同时假手术组和模型组给予等量的蒸馏水。给药7天后对大鼠进行第2次神经功能评分,处死后检测各组大鼠脑梗死体积、梗死周边血管内皮生长因子受体2(VEGFR2)表达及新生血管数量的情况。结果显示,缬芎滴丸能显著改善大鼠脑缺血再灌注损伤后神经功能,减小脑梗死体积,促进脑梗死周边组织VEGFR2表达和新生血管形成。结果表明,缬芎滴丸对大鼠脑缺血再灌注损伤具有保护作用,其机制可能与促进血管新生有关。This study was designed to detect the impact of Valerian Ligusticum Pill （VLP） on cerebral ischemia reperfusion injury in rats, and explore the mechanism of angiogenesis. Sixty SD male rats were randomly divided into five groups, including sham operation group, model group, VLP-low （30 mg ·kg-l） group, VLP-high （50 mg·kg-l） group and nimodipine （10 mg·kg-1） group. The ischemia reperfusion injury model was induced by occlusion of middle cerebral artery with suture embolus, reperfusion after 30 minutes＇ ischemia. When the rats were awake, the first neurological function scores was determined with modified neurological severity score （mNSS）. The rats were given VLP （30 mg·kg 1, 50 mg·kg-1） and nimodipine （10 mg·kg-1） through intragastric administration at 2 mL, once a day for a total of 7 days, while an equal amount of distilled water was used in the sham operation group and model control group. After 7 days, the rats were given second neurological function scores, and improvement of neurological function = [the first score] - [the second score]. The rats were sacrificed to investigate the infarction volume percentage with 2,3,5-triphenyl tetrazolium chloride method; do the qualitative and half quantitative analyses for protein vascular endothelial growth factor receptor 2（VEGFR2） in the tissue of cortex infarction around by Western blot; detect the new blood vessels of cortex infarction around by ki67/lectin immunofluorescence double staining method. Results suggest that VLP could significantly improve the neurological function, reduce the percentage of infarct volume, increase the expression of VEGFR2 and number of new blood vessels in the cortex infarction around compared with model group. In conclusion, VLP may relive the acute cerebral ischemia reperfusion injury in rats by inducing angiogenesis.

关 键 词：脑缺血再灌注 缬芎滴丸 神经功能评分 2 3 5-氯化三苯基四氮唑 血管内皮生长因子受体2 新血管形成 

分 类 号：R966[医药卫生—药理学]