EB病毒脘氧核糖核酸检测在小儿传染性单核细胞增多症的临床应用  

Clinical Application of EB Virus DNA Detection in Children with Infectious Mononucleosis

作　　者：宋宇[1] 李娅[1] 苏艳丹[1] 马晓波[1] 段勇[1] 

机构地区：[1]昆明医科大学第一附属医院,云南昆明650032

出　　处：《黑龙江医学》2016年第8期753-755,共3页Heilongjiang Medical Journal

摘　　要：目的探讨EB病毒(epstein-barrvirus,EBV)脘氧核糖核酸检测在小儿传染性单核细胞增多症的临床应用。方法收集36例传染性单核细胞增多症患儿临床资料,另以40例健康儿童为对照组,应用实时荧光定量聚合酶链式(PCR)检测EB病毒DNA(EBV-DNA),并进行相关统计学分析。结果传单组EBV-DNA阳性率为86.1%,对照组为5.0%,P=0.000;随着EBVDNA拷贝数量增加,ALT、AST、CK、肌酸激酶同工酶(CK-MB)量随之增加、肝脾肿大发生率也随之增加,P<0.05;随着患儿热程延长,EBV-DNA含量呈下降趋势,P<0.05;患儿EBV-DNA首次检测载量与病程呈正相关,r=0.673,P=0.006。结论 EBVDNA早期检测对患儿传染性单核细胞增多症的诊断有意义,对患儿病情判定和预后也有一定价值。Objective To investigate the clinical application of EB virus DNA in the treatment of infectious mononucleosis of children.Methods The clinical data of 36 children with infectious mononucleosis was collected and 40 healthy children were regarded as the control group. Real- time fluorescence quantitative PCR detection EBV- DNA was applied. Results The positive rate of EBV- DNA in IM group was higher than that in control group（ 86. 1% vs 5. 0%,P = 0. 000）. With the increase in the number of copies of EBV- DNA,ALT,AST,CK,CK- MB,incidence of hepatosplenomegaly increased（ P〈0. 05）. The content of EBV- DNA decreased with the extension of the fever（ P〈0. 05）. The content of EBV- DNA was positively correlated with the course of disease in children（ r = 0. 673,P =0. 006）. Conclusion The early detection of EBV- DNA has a high value for the diagnosis and disease prediction of infectious mononucleosis in children.

关 键 词：EB病毒 小儿 传染性单核细胞增多症 

分 类 号：R725.1[医药卫生—儿科]