Construction and application of the Synechocystis sp.PCC6803-ftnA in microbial contamination control in a coupled cultivation and wastewater treatment  

作　　者：Yalei Zhang Chunmin Zhang Xuefei Zhou Zheng Shen Fangchao Zhao Jianfu Zhao 

机构地区：[1]State Key Laboratory of Pollution Control and Resource Reuse, Key Laboratory of Yangtze Water Environment of Ministry of Education,Tongji University [2]Water Conservancy Development Research Center, Taihu Basin Authority of Ministry of Water Resources

出　　处：《Journal of Environmental Sciences》2016年第8期174-181,共8页环境科学学报（英文版）

基　　金：supported by the National Key Technologies R&D Program of China(No.2012BAJ25B02)

摘　　要：Inspired by iron fertilization experiments in HNLC（high-nitrate, low-chlorophyll） sea areas,we proposed the use of iron-rich engineered microalgae for microbial contaminant control in iron-free culture media. Based on the genome sequence and natural transformation system of Synechocystis sp. PCC6803, ftn A（encoding ferritin） was selected as our target gene and was cloned into wild-type Synechocystis sp. PCC6803. Tests at the molecular level confirmed the successful construction of the engineered Synechocystis sp. PCC6803-ftn A. After Fe3＋-EDTA pulsing, the intracellular iron content of Synechocystis sp. PCC6803-ftn A was significantly enhanced, and the algae was used in the microbial contamination control system. In the coupled Synechocystis sp. PCC6803-ftn A production and municipal wastewater（MW, including Scenedesmus obliquus and Bacillus） treatment, Synechocystis sp. PCC6803-ftn A accounted for all of the microbial activity and significantly increased from 70% of the microbial community to 95%.These results revealed that while the stored iron in the Synechocystis sp. PCC6803-ftn A cells was used for growth and reproduction of this microalga in the MW, the growth of other microbes was inhibited because of the iron limitation, and these results provide a new method for microbial contamination control during a coupling process.Inspired by iron fertilization experiments in HNLC（high-nitrate, low-chlorophyll） sea areas,we proposed the use of iron-rich engineered microalgae for microbial contaminant control in iron-free culture media. Based on the genome sequence and natural transformation system of Synechocystis sp. PCC6803, ftn A（encoding ferritin） was selected as our target gene and was cloned into wild-type Synechocystis sp. PCC6803. Tests at the molecular level confirmed the successful construction of the engineered Synechocystis sp. PCC6803-ftn A. After Fe3＋-EDTA pulsing, the intracellular iron content of Synechocystis sp. PCC6803-ftn A was significantly enhanced, and the algae was used in the microbial contamination control system. In the coupled Synechocystis sp. PCC6803-ftn A production and municipal wastewater（MW, including Scenedesmus obliquus and Bacillus） treatment, Synechocystis sp. PCC6803-ftn A accounted for all of the microbial activity and significantly increased from 70% of the microbial community to 95%.These results revealed that while the stored iron in the Synechocystis sp. PCC6803-ftn A cells was used for growth and reproduction of this microalga in the MW, the growth of other microbes was inhibited because of the iron limitation, and these results provide a new method for microbial contamination control during a coupling process.

关 键 词：Synechocystis PCC6803-ftnA Municipal wastewater treatment Microbial contaminants 

分 类 号：X703[环境科学与工程—环境工程] X172