槲皮素对颞叶癫痫大鼠学习记忆能力的影响  

Effects of quercetin on the learning and memory ability of temporal lobe epilepsy in rats

作　　者：张军臣[1] 张冉[1] 张广宁[1] 赵万巨[1] 孔庆霞[2] 侯艳丽[3] 

机构地区：[1]济宁医学院附属医院神经外科,272109 [2]济宁医学院附属医院神经内科,272109 [3]山东省内分泌与代谢病研究所,济南250062

出　　处：《中华诊断学电子杂志》2016年第2期131-135,共5页Chinese Journal of Diagnostics(Electronic Edition)

基　　金：山东省自然科学基金面上项目(ZR2011CM017);山东省高等学校科技计划项目(J12LL62);山东省医学科学院院级青年项目

摘　　要：目的探讨槲皮素对颞叶癫痫大鼠学习记忆能力的作用及其机制。方法 45只成年雄性SD大鼠,应用随机数字表法完全随机分为健康组、癫痫组和槲皮素干预组(40 mg/kg),采用氯化锂-匹罗卡品腹腔注射法建立颞叶癫痫模型。Morris水迷宫试验检测大鼠定位航行能力和空间探索能力;试剂盒测定大鼠海马组织总抗氧化能力(T-AOC)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GSH-Px)的水平;荧光定量聚合酶链式反应(PCR)法检测大鼠海马组织转录因子NF-E_2相关因子2(Nrf2)和脑源性神经营养因子(BDNF)mRNA的相对表达量。采用SPSS 18.0统计软件对数据进行统计分析,平台象限潜伏期时间、停留时间、海马组织各酶活性多组间比较采用单因素方差分析,若P<0.05,进一步用最小差值显著法(LSD)进行两两比较。结果各组大鼠水迷宫实验第3,4,5天寻找平台的潜伏期时间分别为,癫痫组(41.4±1.8)s、(41.7±1.9)s、(40.6±2.1)s,健康组(29.5±1.2)s、(22.8±0.4)s、(18.3±1.4)s,槲皮素干预组(31.7±2.6)s、(31.1±1.4)s、(30.2±1.5)s,各组差异有统计学意义(F=123.49,162.49,98.01;P<0.05);各组大鼠在平台所在象限的游泳时间分别为,癫痫组(32.5±5.9)s,健康组(54.3±6.1)s,槲皮素干预组(45.8±5.9)s,差异有统计学意义(F=107.58,P<0.05)。槲皮素干预组大鼠海马组织T-AOC、SOD、CAT和GSH-Px水平均高于癫痫组[(5.5±1.4)U/mg,(3.3±1.1)U/mg;(58.7±13.9)U/mg,(35.3±12.4)U/mg;(15.3±4.6)U/mg,(10.2±4.2)U/mg;(38.7±11.6)U/mg,(23.9±10.3)U/mg],差异有统计学意义(F=99.21,205.37,20.06,92.97;均P<0.05)。抗氧化关键基因Nrf2 mRNA相对表达量分别为,癫痫组(0.25±0.11),健康组(1.00±0.13),槲皮素干预组(0.82±0.15),差异有统计学意义(F=374.74,P<0.05)。BDNF mRNA相对表达量,癫痫组(1.82±0.22),健康组(1.00±0.18),而槲皮素干预组(1.22±0.19),差异有统计学意义(F=323.34,P<0.05)。结论槲皮素干预可提高癫痫大鼠学ObjectiveTo explore the effects and mechanisms of quercetin on the learning and memory ability of temporal lobe epilepsy （TLE） in rats.MethodsFortyfive male Sprague-Dawley rats were randomly grouped into control,TLE model and quercetin treatment group （40 mg/kg）.Lithium-pilocarpine was used to establish TLE rat model by intraperitoneal injection.Morris water maze test was carried out to determine the navigation ability and space exploration ability of rats.The levels of total antioxidant capacity （T-AOC）,superoxide dismutase （SOD）,catalase （CAT） and glutathione peroxidase （GSH-Px） were detected by commercial kits.The Nrf2 and brain-derived neurotrophic factor （BDNF） mRNA levels were quantified with real-time PCR.Escape latency,residence time,enzymatic activities in hippocampal among groups were compared by one-way analysis of variance.ResultsThe escape latency in the Morris water maze test was longer in TLE model group than that in the control group.After removing platform,the percentage of time in the probe quadrant was shorter in TLE group than that in control group（on the third/fourth/fifth day,F=123.49,162.49,98.01;P〈0.05）.Quercetin significantly shortened the escape latency and prolonged the time in probe quadrant of TLE rats （F=107.58,P〈0.05）.The levels of antioxidant indicators T-AOC,SOD,CAT and GSH-Px in quercetin treatment group were higher than those in other two groups（F=99.21,205.37,20.06,92.97;P〈0.05）and Nrf2 mRNA expression was down-regulated in hippocampus of TLE rats,which was reversed by quercetin （F=374.74,P〈0.05）.In addition,the BDNF mRNA was markedly up-regulated in TLE hippocampus,but down-regulated by quercetin （F=323.34,P〈0.05）.ConclusionsQuercetin can increase the learning and memory ability of TEL rats,and the elevated antioxidant levels of hippocampus may contribute to this process.

关 键 词：癫痫颞叶 黄酮类 海马 认知障碍 氧化性应激 

分 类 号：R285.5[医药卫生—中药学]