水稻隐性早熟突变体ref早熟性的遗传分析和基因定位  

Inheritance and gene mapping of recessive earliness in rice( Oryza sativa L.)

作　　者：王士磊[1] 丁正权 黄海祥[1] 

机构地区：[1]嘉兴市农业科学研究院,浙江嘉兴314016

出　　处：《江苏农业学报》2016年第4期721-724,共4页Jiangsu Journal of Agricultural Sciences

基　　金：嘉兴市科技项目(2010AZ2017;2011AZ1021;2012AY1053;2013BZ26001);浙江省公益项目(2013C32028)

摘　　要：为了更好地理解水稻早熟性的遗传机制,对隐性早熟突变体ref进行了遗传分析和基因定位。突变体ref对光周期不敏感,与6个晚熟品种(系)杂交F1代均为晚熟。ref×嘉禾218 F2分离群体抽穗期呈现连续双峰分布,早抽穗和晚抽穗之比符合1∶3的分离比,表明ref早熟性主要受1对隐性早熟基因控制。F2群体中1 005株早熟和晚熟单株组成定位群体,混合基因池分析发现5号染色体上SSR标记RM7302和RM3853与突变体ref早熟基因连锁。构建5号染色体的连锁图谱,进行表型和标记基因型的连锁分析,进一步确认突变体ref早熟基因定位在标记RM7302和RM3853之间18.32 c M的遗传区间内。To uncover the genetic mechanism regulating early flowering in rice,a newly identified mutant ref was genetically analyzed and mapped for earliness genes. R e f was insensitive to photoperiodic response. The F, progenies,derived from ref and six late flowering accessions, expressed late flowering. The heading date in ref×Jiahe218 F2 population displayed continuous and bimodal frequency distributions,and the ratio early flowering plants to late flowering plants followed a 1-3 segregation ratio, suggesting that earliness was controlled by a pair of recessive genes. A total of 1 005 early/late flowering plants derived from F2 generation was selected to construct the gene mapping population. Bulked segregation analysis identified SSR markers RM7302 and RM3853 on chromosome 5 associated with earliness genes. With further linkage analysis,earliness gene was finally mapped to an interval of 18. 32-cM region between SSR markers RM7302 and RM3S53 on the genetic map of chromosome 5.

关 键 词：水稻 隐性早熟突变体 抽穗期 遗传分析 基因定位 

分 类 号：S511.032[农业科学—作物学]