不同植物叶片角质膜分离方法对角质膜水分渗透性的影响  

Water permeability of plant cuticular membranes isolated with different chemical agents

作　　者：李余 石辉[1] 段保正 魏小芳[1] 蒋子银 

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055

出　　处：《江苏农业学报》2016年第4期769-773,共5页Jiangsu Journal of Agricultural Sciences

基　　金：陕西省自然科学基金重点项目(2014JZ011);林业公益性行业科研专项(201304301-05)

摘　　要：为了探究草酸/草酸铵、果胶酶/纤维素酶、氯化锌/盐酸、三氧化铬4种叶片角质膜分离方法对角质膜水分渗透性的影响,以大叶女贞(Ligustrum lucidum Ait.)、大叶黄杨(Euonymus japonicus)、海桐(Pittosporum tobira)叶片上表皮无气孔角质膜为材料,利用扫描电镜和微蒸腾室分别对角质膜结构与水分渗透速率进行研究。结果表明,与完整叶片对照相比,草酸/草酸铵法剥离出的3种叶片角质膜结构较为完整,且水分渗透速率与完整叶片相近,为最佳的叶片角质膜剥离方法;植物叶片质地的差异影响角质膜剥离方法的选择,纸质叶可优先选择氯化锌/盐酸法、草酸/草酸铵法,厚革质叶片角质膜的剥离可优先选择草酸/草酸铵法。Four chemical agents,oxalic acid / ammonium oxalate solution,cellulase / pectinase solution,zinc chloride-hydrochloric acid solution and chromium trioxide solution were applied to isolate the upper astomatous cuticular membranes of Ligustrum lucidum Ait,Euonymus japonicus and Pittosporum tobira to study the structure of plant cuticles with scanning electron microscope and the speed of water infiltration of cuticular membranes in a micro-transpiration chamber.Compared with unisolated leaf,the structure of plant cuticles was relatively integrated when isolated with oxalic acid / ammonium oxalate solution,and the rate of water infiltration was close to that of complete leaf,which made oxalic acid / ammonium oxalate solution the preferred agent for isolation. Leaf texture was associated with the selection of isolation agents. The zinc chloride-hydrochloric acid solution and oxalic acid / ammonium oxalate solution were the choice for cuticular membranes isolation of papery leaves,and oxalic acid / ammonium oxalate solution was the choice for thick and leathery leaves.

关 键 词：角质膜 分离方法 微蒸腾室 水分渗透速率 

分 类 号：S688[农业科学—观赏园艺]