结球甘蓝幼苗耐热性鉴定方法及耐热生理  

Identification of heat tolerance in cabbage seedlings and heat-tolerant physiology

作　　者：闫圆圆[1] 曾爱松[1] 宋立晓[1] 严继勇[1] 

机构地区：[1]江苏省农业科学院蔬菜研究所,江苏南京210014

出　　处：《江苏农业学报》2016年第4期885-890,共6页Jiangsu Journal of Agricultural Sciences

基　　金：江苏省农业科技支撑计划项目(BE2013429);江苏省农业科技自主创新基金项目[CX(15)1015]

摘　　要：为了筛选出一套适用于甘蓝苗期耐热性鉴定的方法,以耐热性不同的6份甘蓝材料为试验材料,通过采用不同的胁迫温度、胁迫时间和胁迫苗龄对甘蓝苗期耐热性进行评价,并测定不同材料的生理指标。结果显示,4-5片真叶的甘蓝幼苗在380℃/290℃（昼/夜）人工模拟气候箱中处理3 d,可以准确评价不同类型甘蓝材料的耐热性差异。高温处理3 d后,甘蓝幼苗相对电导率大幅升高,其中耐热材料启夏的增幅小于热敏感材料W410。随着高温胁迫时间的延长,启夏和W410的MDA含量和游离脯氨酸含量呈升高的趋势,且两者间差异达显著水平,两种类型材料的SOD活性均呈现出先上升后下降趋势,其中启夏的SOD活性始终高于W410。POD活性变化与耐热性无明显相关性。因此,相对电导率、MDA含量、游离脯氨酸含量和SOD活性均可以作为甘蓝苗期耐热性鉴定的间接指标。To develop a method for identifying heat tolerance at seedling stage,six cabbage varieties or inbred lines with different heat tolerances were evaluated for the optimal temperature,time duration and seedling age of high temperature stress. The cabbage seedlings with 4-5 true leaves inside artificial climate box under 38. 0 ℃ / 29. 0 ℃( d / n) for 3 d was favorable for heat tolerance identification. After high temperature treatment for 3 d,the relative conductivity of cabbage seedlings dramatically increased,and the increment was bigger in heat-sensitive materials than that in heat-tolerant materials. As high temperature stress lasted,the contents of MDA and proline in both heat-tolerant and-sensitive material climbed up,and the SOD activities declined following an initial rising. The SOD activity in heat-tolerant variety was higher than that in heat-sensitive variety. The POD activity was variety-independent. In sum,relative conductivity,MDA content,proline content activity could be used as indirect indicators for heat tolerance identification of cabbage seedlings.

关 键 词：甘蓝 耐热性鉴定 相对电导率 MDA含量 游离脯氨酸含量 SOD活性 

分 类 号：S635.1[农业科学—蔬菜学]