柚皮素对去卵巢所致大鼠骨质疏松症的影响  

Effects of naringenin on osteoporosis in ovariectomized rats

作　　者：吴新涛[1] 石晶[1] 高乐才[1] 孙国昌 吴文元[1] 庞石磊 

机构地区：[1]河北省沧州中西医结合医院骨科,061001 [2]河北省沧州市南大港医院骨科

出　　处：《河北医药》2016年第17期2575-2578,共4页Hebei Medical Journal

基　　金：河北省中医药管理局科研计划项目(编号:2016267)

摘　　要：目的观察柚皮素对去卵巢所致大鼠骨质疏松症的治疗作用。方法 50只雌性SD大鼠随机分为假手术组、模型组、柚皮素低、中、高剂量组,每组10只。假手术组施行假手术,其余4组均行卵巢摘除术,术后4周开始灌胃给予35、70、140 mg/kg柚皮素。12周后分别检测5组大鼠血清雌二醇(E2)、孕激素(P)、抗酒石酸酸性磷酸酶(TRACP)、碱性磷酸酶(ALP)、骨钙素(BGP),尿液钙(Ca)、磷(P)及脱氧吡啶啉(DPD)、骨密度(GMD)、股骨三点弯曲应力、L5腰椎抗压缩载荷及骨小梁形态变化。结果与假手术组比较,模型组大鼠体重明显增加,子宫脏器系数明显降低,差异有统计学意义(P<0.05);柚皮素低、中、高剂量组大鼠体重较模型组明显降低,子宫脏器系数较模型组明显增加,差异有统计学意义(P<0.05),并呈剂量依赖性。与假手术组比较,模型组大鼠血清E2、P明显降低,差异有统计学意义(P<0.05);柚皮素低、中、高剂量组大鼠血清E2、P较模型组明显增加,差异有统计学意义(P<0.05),呈剂量依赖性。与假手术组比较,模型组大鼠血清TRACP及尿液Ca、P、DPD明显增加,差异有统计学意义(P<0.05);柚皮素低、中、高剂量组血清TRACP及尿液Ca、P、DPD较模型组明显降低,差异有统计学意义(P<0.05),且呈剂量依赖性。与假手术组比较,模型组大鼠血清ALP明显增加,BGP明显降低,差异有统计学意义(P<0.05);柚皮素低、中、高剂量组大鼠血清ALP较模型组明显降低,BGP较模型组明显增加,差异有统计学意义(P<0.05),呈剂量依赖性。与假手术组比较,模型组大鼠BMD明显降低,差异有统计学意义(P<0.05);柚皮素低、中、高剂量组大鼠BMD较模型组明显增加,差异有统计学意义(P<0.05),呈剂量依赖性。与假手术组比较,模型组大鼠股骨三点弯曲应力、L5腰椎抗压缩载荷明显降低,差异有统计学意义(P<0.05);柚皮素低、中、高剂量组大鼠股骨三点弯曲应力、L5Objective To evaluate the therapeutic effects of naringenin on osteoporosis in ovariectomized rats.Methods Fifty female SD rats were randomly divided into sham-operation group,model group,low-dose naringenin group,median-dose naringenin group and high-dose naringenin group,with 10 rats in each group. Except for the rats in shamoperation group,the rats in the other 4 groups underwent ovariectomy. On 4 weeks after operation,the rats in low-dose naringenin group,median-dose naringenin group and high-dose naringenin group were given naringenin by gavage,with the dosage of 35 mg / kg,70 mg / kg,140 mg / kg,respectively for 12 weeks. Then the serum levels of estradiol（ E2）,progesterone（ P）,tartrate resistant acid phosphatase（ TRACP）,bone alkaline phosphatase（ ALP）,osteocalcin（ BGP）,urine calcium（ Ca）,phosphorus（ P） and deoxypyridinoline（ DPD）,bone mineral density（ BMD）,the maximum bending stress,loading force of the 5th lumbar vertebra and morphologic changes of bone trabecula were detected and compared among the 5 groups.Results As compared with that in sham-operation group,the body weight in model group was obviously increased,however,uterus organ coefficient was significantly decreased（ P 0. 05）. The body weight in the three medication groups was obviously decreased,however,uterus organ coefficient was significantly increased,as compared with that in model group（ P 0. 05）,with a dose-dependent manner. As compared with those in sham-operation group,the serum levels of E2 and P in model group were significantly decreased（ P 0. 05）. However the serum levels of E2 and P in the three medication groups were significantly increased,as compared with those in model group（ P 0. 05）,with a dose-dependent manner. As compared with those in sham-operation group,the serum levels of TRACP,Ca,P,DPD in model group were significantly increased（ P 0. 05）,however,which in the three medication groups were significantly decreased,as compared with that in model group（ P 0. 05）,wit

关 键 词：柚皮素 去卵巢 骨质疏松 

分 类 号：R681.4[医药卫生—骨科学]