夏枯草茎叶中三萜类成分研究  

Study on the triterpenoid constituents in herb of Prunella vulgaris

作　　者：蔡凡[1] 严启新 

机构地区：[1]深圳市龙岗区妇幼保健院药剂科,广东深圳518172 [2]深圳市海王生物工程股份有限公司,广东深圳518057

出　　处：《广东药学院学报》2016年第4期428-430,共3页Academic Journal of Guangdong College of Pharmacy

摘　　要：目的研究夏枯草茎叶中的化学成分。方法采用正反相硅胶、Sephadex LH-20凝胶、ODS柱层析等手段,对夏枯草茎叶体积分数80%乙醇提取物的乙酸乙酯部位进行分离纯化,再采用紫外、红外、质谱法进行结构鉴定。结果从夏枯草茎叶中分离得到7个乌苏烷型三萜类化合物：乌苏酸（1）、1β,3β-二羟基乌苏烷-12烯-28-酸（2）、2α,3β-二羟基乌苏烷-12烯-28-酸（3）、3-O-α-L-阿拉伯吡喃糖-19α-羟基-乌苏烷-12烯-28-酸（4）、3β,23-二羟基-乌苏烷-12烯-28-酸（5）、2α,3β,19α,23β-四羟基乌苏烷-12烯-28-酸（6）、3α,19α,23,24-四羟基-乌苏烷-12烯-28-酸（7）。结论化合物2、4-7为首次从该植物中分离得到。Objective To study the chemical constituents of the herb of Prunella vulgaris.Methods The ethyl acetate part of 80% ethanol extract from the herb of Prunella vulgaris was isolated by silica gel chromatography,LH-20, basin chromatography, and the structures were identified by UV, IR and NMR.Results Seven triterpenoids were isolated and identified as ursolic acid（ 1）,1β,3β-dihydroxyurs-12-en-28-oic acid（ 2）,2α,3β-dihydroxyurs-12-en-28-oic acid（ 3）,3-O-α-L-arabinophyranose-19α-hydroxylurs-12-en-28-oic acid（ 4）,3β,23-dihydroxyurs-12-en-28-oic acid（ 5）,2α,3β,19α,23β-tetrahydroxyurs-12-en-28-oic acid（ 6）,3α,19α,23,24-tetrahydroxyurs-12-en-28-oic acid（ 7）.Conclusion Compounds 2,4-7 were isolated from Prunella vulgaris for the first time.

关 键 词：夏枯草 化学成分 乌苏烷型三萜类化合物 

分 类 号：R284[医药卫生—中药学]