赤芍总苷对自发性高血压大鼠血小板sCD4OL和炎症因子的干预作用  

Effect of total paeony glycoside on platelet function and soluble CD40L and inflammatory factors expression in spontaneously hypertension rats

作　　者：肖文豪[1] 王卓[2] 李瑞莹[3] 李国标[4] 林忠伟[4] 

机构地区：[1]广东药科大学附属第一医院风湿科,广东广州510080 [2]中山大学附属第一医院病理科,广东广州510080 [3]广东药科大学附属第一医院检验科,广东广州510080 [4]广东药科大学附属第一医院心内科,广东广州510080

出　　处：《广东药学院学报》2016年第4期481-484,共4页Academic Journal of Guangdong College of Pharmacy

基　　金：广东省中医药局科研课题立项项目(20142086)

摘　　要：目的探讨赤芍总苷(TPG)对自发性高血压大鼠(SHR)血小板功能及血清s CD4OL水平和炎症因子的干预作用。方法 8周龄雄性SHR40只随机分为赤芍高、低剂量组、阿司匹林组、生理盐水对照组,另设Wistar大鼠作为正常对照组,连续灌胃给药8周。实验结束后,取全血检测白细胞计数、血小板计数、平均血小板容积(MPV)、血浆凝血酶原时间、血脂水平;取血清,散射比浊法检测hs-CRP,ELISA法测定可溶性CD40L(s CD40L)和一氧化氮(NO)水平。结果血清胆固醇及三酰甘油水平、白细胞总数、血浆凝血酶原时间、血小板计数在5组间比较,差异无统计学意义(P>0.05);SHR大鼠MPV、s CD40L及hs-CRP水平升高,血清NO水平明显下降,与正常对照组比较,差异均有统计学意义(P<0.05);TPG高剂量组MPV、s CD40L、hs-CRP水平降低,NO水平升高,与生理盐水对照组比较,差异有统计学意义(P<0.05)。相关性分析显示,SHR组MPV与血清hs-CRP、s CD40L水平呈正相关(r=0.36,P<0.05;r=0.48,P<0.01);hs-CRP与s CD40L水平呈正相关(r=0.31,P<0.05)。结论赤芍总苷能降低SHR大鼠s CD40L、hs-CRP水平,提高NO水平,可作为高血压治疗的辅助方法。Objective To investigate the effect of total paeony glycoside（ TPG） on platelet function and the level of soluble CD40 L and inflammatory factors in spontaneously hypertension rats（ SHR）.Methods 40 male SHRs,at 8-week-old,were randomly divided into four groups,including the low and high doses of TPG groups,the aspirin group and the saline group.Wistar rats（ n = 8） were also designed as the control group.After intragastric administration 8 weeks,the count of white blood cell（ WBC） and platelet,mean platelet volume（ MPV）,clotting time and lipid levels were measured.The level of serum high sensitivity C-reactive protein（ hs-CRP）,s CD40 L and nitric oxide（ NO） were also examined by scatter turbidimetry and ELISA,respectively.Results There was no significant difference on the levels of serum cholesterol and triglyceride,the counts of WBC and platelet and clotting time in these five groups.Compared with the control group,MPV and the levels of s CD40 L and hs-CRP,but not NO,were increased in the saline group.Compared with the saline group,treatment with the high dose of TPG obviously decreased MPV and the levels of s CD40 L and hs-CRP,but incrased NO level in SHRs.The correlation between MPV and the levels of hs-CRP and s CD40 L was significant（ r = 0.36,P 〈0.05; r = 0.48,P 〈0.01）.The correlation between the level of hs-CRP and s CD40 L was significant（ r = 0.31,P 〈0.05）.Conclusion TPG can reduces the levels of serum hs-CRP and s CD40 L,which may be used for the adjuvant therapy in hypertension.

关 键 词：赤芍总苷 自发性高血压大鼠 血小板 可溶性CD40L 炎症因子 

分 类 号：R285.5[医药卫生—中药学]