MicroRNA在辅助诊断系统性红斑狼疮中的价值  

Role of microRNA in auxiliary diagnosis of systemic lupus erythematosus

作　　者：吴纯[1] 高素荣[1] 董梅[1] 

机构地区：[1]解放军第309医院检验科,北京100091

出　　处：《解放军医学院学报》2016年第8期849-852,共4页Academic Journal of Chinese PLA Medical School

摘　　要：目的探讨micro RNA-146a在辅助诊断系统性红斑狼疮(systemic lupus erythematosus,SLE)中的价值。方法利用免疫印迹法、间接免疫荧光法、茎环RT实时荧光定量PCR(RT-q PCR)检测2014年9月-2015年2月在我院内分泌科住院的33例SLE患者、21例非SLE自身免疫性疾病对照组患者和24例健康体检对照组人血浆中抗可提取性核抗原(extractable nuclear antigen,ENA)、抗核抗体(antinuclear antibody,ANA)和micro RNA-146a的表达水平,比较3组间检测指标的差异;SLE患者稳定期与活动期micro RNA-146a的表达差异,micro RNA-146a与ANA检测水平的相关性。结果 SLE患者活动期micro RNA-146a相对表达量(relative expression,RE)高于稳定期(276.24±255.93 vs 102.76±59.55,P<0.05);SLE组micro RNA-146a的RE为223.67±229.31,高于疾病对照组(10.38±4.49)和正常对照组(4.68±3.18)(P<0.01),两对照组间差异无统计学意义;micro RNA-146a的表达量与ANA滴度无相关性(r=0.043、P=0.801)。结论 micro RNA-146a可以作为一个SLE的潜在诊断标记物,在判断SLE病情的严重程度、监测疾病的发生发展及恢复状况时有参考意义。Objective To investigate the role and clinical value of micro RNA-146 a in auxiliary diagnosis of systemic lupus erythematosus（SLE）. Methods Western Blot method, indirect immunofluorescence method, stem ring RT real-time fluorescence quantitative PCR（RT-q PCR） were used to detect anti-ENA（Extractable Nuclear Antigen）, ANA（antinuclear antibody） and micro RNA-146 a level in plasma of 33 SLE patients, 21 non-SLE patients in disease control group and 24 healthy cases in control group, the differences of detection index between three groups were compared; micro RNA-146 a expression differences of patients in stable or active stage, and the correlation between detection level of ANA and micro RNA-146 a were also compared. Results For SLE patients, the relative expression（RE） of micro RNA-146 a in active subgroup was higher than inactive subgroup [（276.24±255.93） vs（102.76±59.55）, P〈0.05]; and the micro RNA-146 a RE of SLE group was higher than disease control group and normal control group [（223.67±229.31） vs（10.38±4.49）,（4.68±3.18）, P〈0.01], while there was no statistical difference between two control groups. The correlation coefficient of level of micro RNA-146 a and ANA was r=0.043, P=0.801.Conclusion micro RNA-146 a can be used as a potential diagnostic marker of SLE, and it has important value in judging the severity of SLE and monitoring disease onset, development and the status of recovery.

关 键 词：小分子RNA 系统性红斑狼疮 小分子RNA-146a 

分 类 号：R593.241[医药卫生—内科学]