巴曲酶早期应用对大鼠脑外伤后神经功能恢复的促进作用  

Neurorestorative effects of Batroxobin in early treatment on traumatic brain injury in rats

作　　者：苏辉[1] 戴宜武[1] 邹德非[1] 白妙春[1] 

机构地区：[1]北京军区总医院附属八一脑科医院神经外科,北京100700

出　　处：《解放军医学院学报》2016年第8期879-883,共5页Academic Journal of Chinese PLA Medical School

摘　　要：目的观察巴曲酶对大鼠创伤性脑损伤(traumatic brain injury,TBI)后神经功能的保护作用。方法本实验采用改良的大脑皮质撞击法制作成年雄性大鼠TBI模型。20只成年大鼠随机分成2组:0.9%氯化钠注射液组(n=10)和巴曲酶组(n=10)。术后30 min,分别经尾静脉注射巴曲酶(2 U/kg)或0.9%氯化钠注射液治疗,1次/d,连续30 d。采用改良大鼠神经功能缺损评分(modified neurological severity score,m NSS)、横梁平衡实验(beam balance test,BBT)、横梁爬行实验(beam walking test,BWT)、Gridwalk实验和Morris水迷宫实验(Morris water maze test,MWMT)分别对大鼠感觉运动功能、空间探索和学习能力进行评估。结果第7天m NSS:巴曲酶组5.5±0.8,0.9%氯化钠注射液组7.5±0.9(P<0.05);BBT第14天平衡维持时间:巴曲酶组(34.8±5.9)s,0.9%氯化钠注射液组(19.2±4.2)s(P<0.01);BWT第7天横梁爬行时间:巴曲酶组(42.0±4.5)s,0.9%氯化钠注射液组(53.1±5.1)s(P<0.05);Gridwalk第7天前肢足误比例:巴曲酶组8.8%±1.9%,0.9%氯化钠注射液组14.3%±2.1%(P<0.01);MWMT第32天潜伏时间:巴曲酶组(30.3±2.5)s,0.9%氯化钠注射液组(38.6±2.3)s(P<0.05)。结论本实验首次发现早期应用巴曲酶能够显著促进TBI大鼠神经功能恢复,可作为TBI治疗的潜在药物。Objective To investigate the neurorestorative effects of Batroxobin on traumatic brain injury（TBI） in rats. Methods TBI model was induced by controlled cortical impact in adult male rats. Twenty young adult rats were randomly divided into two groups： saline-treated group（n=10） and Batroxobin-treated group（n=10）. Thirty minutes after TBI, all rats were treated with Batroxobin（2 U/kg） q.d or saline q.d by caudal vein injection for 30 consecutive days. Sensorimotor function and spatial learning were assessed using modified neurological severity score（m NSS）, beam balance test（BBT）, beam walking test（BWT）, Gridwalk test and the morris water maze test（MWMT）, respectively. Results The m NSS at 7 days after surgery was（5.5±0.8） in Batroxobin group and（7.5±0.9） in saline group（P〈0.05）. Balance latency in BBT at 14 days after surgery was（34.8±5.9） s for Batroxobin group and（19.2±4.2） s for saline group（P〈 0.01）. Escape latency in BWT at 7 days after surgery was（42.0±4.5） s for Batroxobin group and（53.1±5.1） s for saline group（P 〈0.05）. The percentage of forelimb foot faults in Gridwalk test at 7 days after surgery was（8.8%±1.9%） for Batroxobin group and（14.3%±2.1%） for saline group（P〈 0.01）. Escape latency in MWMT at 32 days after surgery was（30.3±2.5） s for Batroxobin group and（38.6±2.3） s for saline group（P 〈0.05）. Conclusion Our study demonstrate that early administration of Batroxobin significantly improves functional outcomes in rats with TBI for the first time, which indicates that Batroxobin has considerable therapeutic potential for patients with TBI.

关 键 词：巴曲酶 脑创伤 神经功能评估 大鼠 

分 类 号：R651.15[医药卫生—外科学]