Effects of Salt Stress on Anatomical Structure of Leaves of Malus sieversii and Malus robusta  

盐胁迫对八棱海棠和新疆野苹果叶片解剖结构的影响（英文）

作　　者：马兰[1] 吴玉霞[1] 何天明[1] 

机构地区：[1]新疆农业大学林学与园艺学院,新疆乌鲁木齐830052

出　　处：《Agricultural Science & Technology》2016年第8期1777-1779,1785,共4页农业科学与技术（英文版）

基　　金：国家公益性行业(农业)专项(20130309305);国家自然科学基金项目(31160382)~~

摘　　要：[Objective] This study was conducted to compare leaf anatomical structure of Malus sieversii and M. robusta under different salt concentration levels to determine their tolerance to salt and thus to provide rootstock materials for apple production in salinized soil in Southern Xinjiang. [Method] The experiment was conducted with M. sieversii and M. robusta as test materials. Salt stress was simulated using 8 g/L of NaCI solution, and Hoagland nutrient solution was used instead of NaCI solution as control group （CK）. Samples were collected on the 20^th d of treatment, sliced through paraffin processing. The prepared paraffin sections of M. sieversii and M. robusta were then observed under a light microscope for anatomical structures of leaf, upper epidermis, lower epidermis, palisade tissue and spongy tissue. [Result] Compared with the control, the leaf, upper epidermis, lower epidermis and spongy tissue of M. sieversii and M. robusta under salt stress were thickened at different degrees, while the thickness of the palisade tissue was decreased. Moreover, high salt concentration caused severer damage to the cell structure of M. sieversii than to that of M. robusta, as M. robusta cells maintained better structural integrity. [Conclusion] M. robusta has higher adaptability to salt stress than M. sieversii.[目的]比较八棱海棠和新疆野苹果在不同盐浓度下叶片组织解剖结构的变化,分析其耐盐性,为我区南疆盐渍化土壤条件下苹果的商业化提供砧木资源。[方法]试验以盆栽的八棱海棠和新疆野苹果为试验材料,用8 g/L的NaCl溶液进行盐胁迫处理,以只浇灌Hoagland完全营养液作为对照(CK),在处理第20天取样,采用石蜡制片法制作切片,测量出八棱海棠和新疆野苹果的叶片、上表皮、下表皮、栅栏组织和海绵组织指标,观察分析八棱海棠和新疆野苹果叶片的解剖结构。[结果]光镜显微结构表明,与对照相比,在盐胁迫环境下,八棱海棠和新疆野苹果的叶片厚度、上下表皮、海绵组织发生不同程度的加厚,而栅栏组织厚度减小。与新疆野苹果相比,8 g/L NaCl处理的八棱海棠叶片细胞结构损伤较小,能够较好地保持细胞结构的完整性。[结论]八棱海棠对盐胁迫的适应能力高于新疆野苹果,对盐胁迫表现出较强的适应性。

关 键 词：NaCl stress M. sieversii M. robusta Leaf anatomical structure 

分 类 号：S661.1[农业科学—果树学]