UPLC-Q-TOF-MS/MS快速分析苁蓉总苷胶囊化学成分  

Rapid identification of chemical components in Congrong Zonggan capsule by UPLC-Q-TOF-MS/MS

作　　者：吴金凤[1,2] 尹权微 潘有智[2,3] 王雪[2,3] 秦建平[2,3] 李家春[2,3] 黄文哲[2,3] 王振中[1,2,3] 

机构地区：[1]南京中医药大学,江苏南京210023 [2]江苏康缘药业股份有限公司,江苏连云港222001 [3]中药制药过程新技术国家重点实验室,江苏连云港222001

出　　处：《中国中药杂志》2016年第17期3244-3251,共8页China Journal of Chinese Materia Medica

基　　金：国家"重大新药创制"科技重大专项(2013ZX09402203)

摘　　要：该文采用超高效液相色谱-四极杆飞行时间高分辨率质谱(UPLC-Q-TOF-MS/MS)技术对苁蓉总苷胶囊中化学成分进行了定性分析。液相条件:Agilent SB-C_(18) Rapid Resolution HD(3.0 mm×100 mm,1.8μm)色谱柱,以乙腈-0.1%甲酸水溶液为流动相进行梯度洗脱,体积流量0.2 m L·min-1,柱温30℃,检测波长330 nm;质谱条件:采用ESI源,负离子模式下进行数据采集。通过与对照品对比、数据库匹配分析及参考文献资料,共推测出苁蓉总苷胶囊中的41个化合物。该方法能快速检测苁蓉总苷胶囊的化学成分,为苁蓉总苷胶囊的质量控制提供参考,也为其药效物质基础的进一步研究奠定基础。This study was aimed to qualitatively analyze the chemical components in Congrong Zonggan capsule by using ultra-per- formance liquid chromatography tandem quadrupole time-of-flight mass spectrometry method （UPLC-Q-TOF-MS/MS）. An Agilent SB-C18 Rapid Resolution HD （3.0 mm × 100 mm,1.8 μm） was used with acetonitrile （A） - 0. 1% formic acid solution （B） as the mobile phase for gradient elution. The flow rate was 0. 2 mL · min^-1 ; the detection wavelength was set at 330 nm and the column temperature was maintained at 30 ℃. Electrospray ion （ESI） source was applied for the qualitative analysis under the negative ion mode. Finally, based on comparison with standard samples, database matching analysis and reviewing relevant literature, 41 compounds were identified from Con- grong Zonggan capsule. This method could be used to rapidly detect the chemical components in Congrong Zonggan capsule, providing refer- ence for the quality control of Congrong Zonggan capsule and laying a foundation for the further study on active components mechanism.

关 键 词：苁蓉总苷胶囊 UPLC-Q-TOF-MS/MS 化学成分 质谱特征 裂解规律 

分 类 号：R286.0[医药卫生—中药学] O657.63[医药卫生—中医学]