原多甲藻酸(AZA1)对Hep-G2细胞毒理作用的研究  

Study on the toxicological effect of azaspiracid-1 (AZA1) on Hep-G2 cells

作　　者：孙晓飞[1] 宋大贺 丁健[1] 刘淑艳[1] 蒋丹[1] 林长军[1] 

机构地区：[1]辽宁出入境检验检疫局,辽宁大连116001 [2]大连机场出入境检验检疫局,辽宁大连116033

出　　处：《黑龙江畜牧兽医》2016年第9期184-186,189,297,共5页Heilongjiang Animal Science And veterinary Medicine

基　　金：国家质检总局质检公益性行业科研专项(201410059);国家质检总局科技计划项目(2013IK037)

摘　　要：为了研究原多甲藻酸(AZA1)的毒理作用,试验以Hep-G2细胞为作用对象,采用CCK-8细胞增殖-毒性检测试剂盒测定吸光度值,对不同浓度原多甲藻酸的毒理作用进行测定。结果表明:当原多甲藻酸浓度达到40 nmol/L时,对细胞毒理作用显现;当浓度达到60 nmol/L时,对细胞毒理作用显著。说明双壳贝类中一定量的原多甲藻酸会对人类健康产生影响。To study the toxicological effect of azaspiracid - 1 （ AZA1 ）, Hep - G2 cells were used as action objects in the test. The toxicological effects of different AZA1 concentrations on Hep - G2 ceils were determined by CCK - 8 cell proliferation and cytotoxity detection Kit for measur- ing absorbance values. The results showed that AZA1 had an obvious toxicological effect on Hep - G2 cells when its concentration reached 40 nmoL/L, while AZA1 had a significant toxicological effect on Hep - G2 cells when its concentration reached 60 nmol/L. The result indicates that a certain amount of AZA1 in bivalves will affect mankind health.

关 键 词：原多甲藻酸(AZA1) Hep-G2细胞 CCK-8 细胞浓度 细胞培养 毒理作用 

分 类 号：S856.9[农业科学—临床兽医学]