金复康“扶助正气、清透伏毒”预防肺癌转移的细胞学机制研究  

The cytologic mechanism of Jin Fu Kang Decoction on preventing lung cancer metastasis by clearing hidden pathogen

作　　者：阙祖俊[1] 罗斌[2] 周之毅[3] 王青[1] 金郁[4] 赵小东[5] 李和根[3] 施奇惠[5] 田建辉[1,3,6] 刘嘉湘[3] 

机构地区：[1]上海市中医药研究院中医肿瘤研究所,上海200032 [2]上海中医药大学研究生院,上海201203 [3]上海中医药大学附属龙华医院肿瘤科,上海200032 [4]华东理工大学药学院,上海200237 [5]上海交通大学系统生物医学研究中心,上海200240 [6]中国中医科学院中医内科学博士后流动站,北京100700

出　　处：《上海中医药杂志》2016年第8期70-74,共5页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(81373621);国家中医药管理局第一批全国中医药传承博士后项目(135818);国家中医药管理局临床研究基地建设专项(JDZX2012125)

摘　　要：目的观察金复康冻干粉(JFK)对非小细胞肺癌(NSCLC)患者的"伏毒"——循环肿瘤细胞(CTC)的清除作用,并验证金复康干预CTC预防转移的假说。方法从肺癌患者外周血中富集并培养CTC细胞系,然后以金复康干预CTC细胞,采用细胞计数试剂盒-8(CCK-8)法检测细胞增殖,并检测克隆形成,以流式细胞术和激光共聚焦技术检测细胞凋亡与周期。结果金复康能够显著以时间和浓度依赖性抑制CTC细胞增殖(P<0.05);金复康可剂量依赖性显著抑制CTC细胞克隆形成(P<0.05);金复康能够有效诱导CTC细胞发生凋亡(P<0.01);高浓度组的金复康能够显著将CTC细胞增殖阻滞在S期(P<0.05)。结论金复康对NSCLC患者体内CTC具有显著的抑制作用,干预CTC可能是JFK防治NSCLC复发与转移的机制。Objective To observe the effect of JinFuKang Decoction （JFK） on clearing the ＂hidden pathogen＂--circulating tumor cells （CTC） in patients with non-small cell lung cancer （NSCLC） and verify the hypothesis that JFK prevent metastasis through intervening CTC. Methods The CTC cell line from peripheral blood in patients with NSCLC were enriched and cultivated, then the CTC cells were intervened by JFK. The Cell proliferation and clone formation were detected by cell counting kit -8 （CCK-8 ）. The Cell apoptosis and cycle were detected by flow cytometry and confocal laser scanning technique. Results The CTC cell proliferation was significantly inhibited by JFK in time-dependent and concentration- dependent manner. The clone formation of CTC cell was significantly inhibited by JFK in a dose-dependent manner（ P 〈 0.05 ）. JFK effectively induced the apoptosis of CTC cells （ P 〈 0.01 ） ; the CTC ceil proliferation was blocked in the S phase in JFK group with high concentration （P 〈 0.05 ）. Conclusion JFK could inhibit the CTC in patients with NSCLC, which indicated that interventing CTC may be the mechanism of JFK on prevention and treatment of the recurrence and metastasis in NSCLC.

关 键 词：金复康口服液 循环肿瘤细胞 非小细胞肺癌 伏毒 

分 类 号：R273[医药卫生—中西医结合]