病证结合复制大鼠脾肾阳虚腹泻型肠易激综合征模型的探讨  

Discussion on the establishment of rat model of diarrhea-predominant irritable bowel syndrome with yang deficiency in spleen and kidney based on the combination of disease and syndrome

作　　者：朱佳杰[1,2] 苏晓兰[1] 刘珊[3] 杨晨 

机构地区：[1]中国中医科学院望京医院脾胃病科,北京100102 [2]北京中医药大学东直门医院脾胃病科,北京100700 [3]北京中医药大学基础医学院,北京100029 [4]北京市密云中医医院内二科,北京101500

出　　处：《上海中医药杂志》2016年第9期15-17,35,共4页Shanghai Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81072800);国家自然科学基金青年基金项目(81403389);中央级公益性科研院所基本科研业务费专项资金资助项目(ZZ0908026)

摘　　要：探讨病证结合脾肾阳虚腹泻型肠易激综合征大鼠模型的建立和评价方法。认为可采用较为公认的母婴分离、直肠扩张、避水应激等方法建立疾病模型,同时以饮食不节、苦寒泻下结合劳倦过度、惊恐伤肾的复合因素复制中医脾肾阳虚证模型;模型评价可从宏观体征、个别理化指标、药物反证和造模因素反推4个维度进行,使得相关模型更符合中医理论。This paper discussed the establishment and evaluation of rat model of diarrhea-predominant irritable bowel （D-IBS） syndrome with yang deficiency in spleen and kidney based on the combination of disease and syndrome. It was held that maternal separation, colorectal distention and water avoidance stress were the accepted methods for establishment of D-IBS model, as the animal model with yang deficiency in spleen and kidney was established by improper diet, diarrhea induced by bitter and cold herbs combined with overstrain and panic;model evaluation could be conducted in the following aspects as macroscopic sign, individual physicochemical index, drug reduction and model factors, which conform to the theory of Chinese medicine.

关 键 词：肠易激综合征 腹泻型 脾肾阳虚 模型 病证结合 

分 类 号：R259[医药卫生—中西医结合] R-332[医药卫生—中医内科学]