高迁移率族蛋白B1对人气道平滑肌细胞增殖和迁移及Akt磷酸化的影响  

Effects of high-mobility group box protein 1(HMGB1) on the proliferation, migration and phosphorylation of Akt in human airway smooth muscle cells

作　　者：柏芳芳 陈子[1] 韩露[1] 闫兆[2] 王楠[1] 解卫平[1] 黄茂[1] 周林福[1,3] 

机构地区：[1]南京医科大学第一附属医院呼吸内科,江苏南京210029 [2]徐州医科大学附属淮海医院呼吸内科,江苏徐州221000 [3]南京医科大学附属江苏盛泽医院呼吸内科,江苏苏州215228

出　　处：《南京医科大学学报（自然科学版）》2016年第7期794-797,共4页Journal of Nanjing Medical University(Natural Sciences)

基　　金：国家自然科学基金(81370133;81170018);江苏省医学重点人才项目(RC2011066);江苏省高校自然科学研究项目(11KJB320008);江苏省临床医学科技专项(BL2012012);江苏省社会发展重点研发专项(BE2015651);江苏省卫生计生委预防医学科研课题(Y2015026);江苏省中医药科技项目(YB2015110);苏州市科技计划项目(SYS201402)

摘　　要：目的：探讨高迁移率族蛋白B1（high morbility group protein box 1,HMGB1）刺激对人气道平滑肌细胞（human airway smooth muscle cells,HASMC）增殖和迁移的影响及其机制。方法：体外培养HASMC,将纯化的第3~8代细胞随机分为对照组、HMGB1组（125、250、500和1 000 ng/m L）、TGF-β阳性对照组（1 ng/m L）,CCK-8法测定HASMC增殖能力,Transwell小室观察HMGB1对HASMC迁移的影响,Western blot检测HASMC的Akt磷酸化。结果 ：与对照组相比,HMGB1呈浓度依赖性刺激HASMC增殖（P〈0.05）;HMGB1（500 ng/m L）刺激HASMC后,细胞迁移能力显著高于对照组（P〈0.01）;HMGB1（500 ng/m L）组HASMC的Akt磷酸化水平较对照组显著增高（P〈0.05）。结论 ：HMGB1促进HASMC增殖和迁移,可能是通过调节PI3K/Akt通路参与气道重塑,为今后治疗哮喘提供了新思路。Objective：To investigate the effects and underlying mechanism of high mobility group protein box 1 （HMGB1） on the proliferation and migration of human airway smooth muscle cells （HASMCs）. Methods：Normal HASMC between passages 3 and 8 were randomly divided into the control group, HMGB1 group with different concentrations（125, 250, 500, and 1 000 ng/mL）, and TGF-β （1 ng/mL） group as a positive control, respectively. The HMGBl-stimulated proliferation of HASMCs was evaluated by cell counting of CCK-8. The number of migrated cells was performed using Transwell migration assay. Western blotting assay was performed to detect the phosphorylation of Akt following HMGB1 stimulation. Result： The proliferation of HASMCs treated with HMGB1 was significantly increased in a dose-dependent manner than those of untreated control group （P 〈 0.05）. Furthermore, the number of migrated cells was significantly increased after HMGB 1 activation compared to the control group（P 〈 0.01）. The phosphorylation level of Akt in the HMGB1 group（500 ng/mL） was significantly increased compared with the control group（P 〈 0.05）. Conclusion： Our data suggest that HMGB1 contributes to airway remodeling by stimulating the proliferation and migration of HASMCs through regulating phosphorylation of PI3K/Akt pathway, which provides a promising target for future therapy of asthma.

关 键 词：高迁移率族蛋白B1 气道平滑肌细胞 增殖 迁移 气道重塑 哮喘 

分 类 号：R562.2[医药卫生—呼吸系统]