更年汤对肾阳虚围绝经期模型大鼠肾上腺皮质中Bax和Bcl-2蛋白表达的影响  

Effect of Gengnian Tang on Bax and Bcl-2 Protein Expression of Adrenal Cortex of Perimenopause Model Rats of Kidney-yang Deficiency

作　　者：武创新[1] 张帆[2] 曹俊岩[2] 齐帅英[3] 易华娅 

机构地区：[1]华亭县人民医院,甘肃华亭744100 [2]贵阳中医学院第二附属医院,贵州贵阳550003 [3]贵阳中医学院,贵州贵阳550002

出　　处：《新中医》2016年第8期303-306,共4页New Chinese Medicine

基　　金：国家自然科学基金项目(81160491)

摘　　要：目的：探讨中药复方更年汤对肾阳虚型围绝经期模型大鼠肾上腺皮质中Bax、Bcl-2表达的影响。方法：建立自然衰老的雌性肾阳虚型围绝经期大鼠模型,11-12月龄60只,随机分为更年汤组、利维爱组、生理盐水组,每组20只;4月龄20只作为青年组;18月龄20只作为老年组。用免疫组化法检测肾上腺皮质Bax、Bcl-2的表达。结果：与生理盐数组比较,利维爱组肾上腺皮质Bax表达降低、Bcl-2的表达升高,差异均有统计学意义（P〈0.05）。更年汤组肾上腺皮质Bax阳性总积分表达低于利维爱组、生理盐水组,Bcl-2阳性总积分表达高于利维爱组、生理盐水组,差异均有统计学意义（P〈0.05）,更年汤组稍优于利维爱组。结论：自拟更年汤能降低肾阳虚型围绝经期模型大鼠肾上腺皮质Bax的表达,提高肾上腺皮质Bcl-2表达水平,平衡围绝经期内分泌失调。Objective：To discuss Chinese medicine（CM）compound Gengnian tang on Bax and Bcl-2 protein expression of adrenal cortex of perimenopause model rats of kidney-yang deficiency syndrome.Methods：Established kidney-yang deficiency female rats model of perimenopause,which was natural aging,herein,60 cases in 11~12 months old.Divided them into Gengnian tang group,livial group and normal saline group randomly,20 cases in each group.Take 20 cases of 4 months old as youth group,20 cases of 18 months old as elderly group.Detected expression of Bax and Bcl-2 of adrenal cortex by immunohistochemical method.Results：Comparing normal saline group,Bax expression of adrenal cortex in livial group was reduced and Bcl-2 expression was increased,differences being significant（P〈0.05）.Bax positive expression total scores of adrenal cortex in Gengnian tang group were lower than those in livial group and normal saline group,Bcl-2 positive expression total scores were higher than those in livial group and normal saline group,differences being significant（P〈0.05）,which in Gengnian tang group was superior to those in livial group.Conclusion：Self-made Gengnian tang can reduce Bax expression of adrenal cortex in perimenopause model rats of kidney-yang deficiency,increase Bcl-2 expression level of adrenal cortex,and balance endocrine dyscrasia in perimenopause.

关 键 词：肾阳虚 围绝经期模型 肾上腺皮质细胞 Bax及Bcl-2 更年汤 动物实验 大鼠 

分 类 号：R285.5[医药卫生—中药学]