昆明地区儿童脑脊液细菌培养阳性结果分析  

Analysis of Positive Culture of Cerebrospinal Fluid in Children of Kunming Area

作　　者：黄海林[1] 王海平[1] 张倩[2] 奎莉越[1] 苏敏[1] 祁洪娟[1] 杜廷义[1] 

机构地区：[1]昆明医科大学附属儿童医院检验科,昆明650228 [2]昆明市妇幼保健院检验科,昆明650021

出　　处：《现代检验医学杂志》2016年第4期151-153,共3页Journal of Modern Laboratory Medicine

基　　金：“昆明地区儿童抗菌药物耐药性监测及临床研究”(云南省科技厅项目,编号:2013FZ241);“肺炎链球菌的病原流行病学监测与分子生物学研究”(昆明市科技局项目,编号:2015-1-S-00564)

摘　　要：目的 监测近年来昆明地区儿童细菌性脑膜炎（BM）病原菌分布特点。方法 对在2012年7月-2015年6月间,昆明医科大学附属儿童医院脑脊液细菌培养阳性结果进行统计,并结合患者的出院诊断及其它实验室相关检测资料进行回顾性分析。结果 4 627例合格的脑脊液培养标本中,阳性标本共计97例,阳性率为2.1%（97/4 627）,其中首次检出细菌为91株（含污染菌17株）。前三位的病原菌依次为大肠埃希菌23株（31.1%）、肺炎链球菌15株（20.3%）和无乳链球菌7株（9.5%）,三者共占检出病原菌的60.8%（45/74）。结论 大肠埃希菌、肺炎链球菌是主要病原菌。实验室应加强污染菌的鉴别以提高临床微生物学实验室服务质量。Objective To investigate the pathogenic distribution characteristics of bacterial meningitis （BM） of the children in the Kunming area. Methods The positive results of Cerebrospinal fluid （CSF） bacterial culture samples in the period of July,2012-June,2015 were statistically analyzed. And a retrospective review was performed combining with the discharge diagnosis as well as other related testing data of these corresponding patients. Results 4 627 cases of qualified CSF samples were cultured,and 97 positive results which belonged to 91 patients were obtained, 17 strains of them were contaminative bacteria,so the contamination rate was 18.7% （17/91）. The top three main BM pathogens in this group were showed orderly as follows ： Escherichia coli 23 （31.1%）, Streptococcus pneumonia 15 （20.3%） and Streptococcus agalactiae 7 （9.5%）, accounted totally for a 60.8% （45/74）. Conclusion For children＇s BMs in this area, the isolates of Escherichia coli and Streptococcus pneumonia were the main pathogens. It is an inevitable request to exclude the interference of contaminative bacteria for the purpose to improve the qualities of our clinical-microbiologic service.

关 键 词：儿童 细菌性脑膜炎 脑脊液 病原菌 

分 类 号：R851.3[医药卫生—航空、航天与航海医学]