复方钩藤降压片对自发性高血压大鼠血管重塑单核细胞趋化蛋白-1和基质金属蛋白酶-9表达的影响  

Effects of Compound Uncaria Hypotensive Tablets on Expressions of MCP-1 and MMP-9 of Vascular Remodeling in Spontaneously Hypertensive Rats

作　　者：冯进[1] 张稳[1] 卿俊[2] 刘惠敏[3] 谭元生[3,4] 

机构地区：[1]湖南中医药大学第一附属医院,湖南长沙410007 [2]湖南省中医药研究院附属医院,湖南长沙410006 [3]湖南中医药大学,湖南长沙410208 [4]湖南省中医药研究院,湖南长沙410208

出　　处：《中国中医药信息杂志》2016年第10期51-55,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81473616);湖南省科技厅重点项目(2013SK2025);湖南省教育厅重点项目(15A142);湖南省中医药管理局重点项目(201403);湖南省中药粉体与创新药物省部共建国家重点实验室开放基金(ZYFT201503)

摘　　要：目的观察复方钩藤降压片对自发性高血压大鼠(SHR)血管重塑单核细胞趋化蛋白-1(MCP-1)、基质金属蛋白酶-9(MMP-9)表达的影响,探讨其可能的作用机制。方法 24只12周龄雄性SHR随机分为模型组、复方钩藤降压片组和阳性药组,每组8只,另设8只WKY大鼠为空白对照组。各给药组给予相应药物灌胃,连续6周。无创尾动脉测压法观测血压;HE染色观察大鼠胸主动脉、肾动脉形态学变化;免疫组化检测胸主动脉内皮细胞MCP-1、MMP-9的蛋白表达。结果模型组胸主动脉壁MCP-1、MMP-9蛋白表达明显高于空白对照组(P<0.01);与模型组比较,各给药组胸主动脉壁MCP-1、MMP-9蛋白表达显著降低(P<0.05,P<0.01);复方钩藤降压片组与阳性药组比较,胸主动脉壁内皮细胞MCP-1、MMP-9蛋白表达无明显差异。结论复方钩藤降压片具有降低SHR血压,减轻炎症反应,调控血管重塑的作用,其机制可能与下调SHR胸主动脉壁内皮细胞MCP-1、MMP-9蛋白表达有关。Objective To observe the effects of Compound Uncaria Hypotensive Tablets on expressions of MCP-1 and MMP-9 of vascular remodeling in spontaneously hypertensive rats（SHR）; To discuss it possible mechanism of action. Methods Totally 24 12-week old male SHR were randomly divided into SHR model group, Compound Uncaria Hypotensive Tablets group, and positive medicine group, with 8 rats in each group. Another 8 WKY rats were set as normal control group. Medication groups were given relevant medicine for gavage for successive 6 weeks. Noninvasive tail cuff method was used to observe blood pressure; morphological changes in thoracic aorta and renal artery were observed by HE staining; immunohistochemistry was used to detect the protein expressions of MCP-1 and MMP-9 in thoracic aortic wall. Results Protein expressions of MCP-1 and MMP-9 in thoracic aortic wall of SHR model group were significantly higher than those of normal control group（P〈0.01）; Compared with the SHR model group, protein expressions of MCP-1 and MMP-9 in thoracic aortic wall decreased significantly in the medication groups（P〈0.05, P〈0.01）; Compared the Compound Uncaria Hypotensive Tablets group and positive medicine group, there was no obvious difference in protein expressions of MCP-1 and MMP-9 in thoracic aortic wall. Conclusion Compound Uncaria Hypotensive Tablets can reduce the blood pressure of SHR, reduce inflammation reaction, and regulate vascular remodeling, which mechanism may be related to down-regulation of expressions of MCP-1 and MMP-9 in SHR aortic endothelial cells.

关 键 词：复方钩藤降压片 自发性高血压大鼠 血管重塑 单核细胞趋化蛋白-1 基质金属蛋白酶-9 

分 类 号：R285.5[医药卫生—中药学]