四神丸对溃疡性结肠炎模型大鼠结肠组织Toll样受体2、4表达的影响  

Effects of Sishen Pills on Expressions of TLR-2 and TLR-4 of Colonic Tissue in Rats with Ulcerative Colitis

作　　者：朱向东[1,2] 王燕[2] 何兰娟[2] 郭婷婷[2] 王迪[2] 

机构地区：[1]中国中医科学院广安门医院,北京100053 [2]甘肃中医药大学,甘肃兰州730000

出　　处：《中国中医药信息杂志》2016年第10期67-71,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(81360541)

摘　　要：目的观察四神丸对脾肾阳虚型溃疡性结肠炎(ulcerative colitis,UC)大鼠结肠组织Toll样受体(TLR)-2、TLR-4基因及蛋白表达的影响,探讨其治疗脾肾阳虚型溃疡性结肠炎的作用机制。方法采用氢化可的松注射液腹腔注射+番泻叶灌胃+三硝基苯磺酸/乙醇灌肠法建立脾肾阳虚型溃疡性结肠炎大鼠模型。将90只Wistar大鼠随机分为空白组、模型组、阳性药组及四神丸高、中、低剂量组,各给药组给予相应药物干预。RT-q PCR检测大鼠结肠组织TLR-2、TLR-4 m RNA表达,Western blot检测大鼠结肠组织TLR-2、TLR-4蛋白表达,并测定血清超氧化物歧化酶(SOD)活性及丙二醛(MDA)含量。结果与空白组比较,模型组大鼠血清SOD活性明显降低、MDA含量明显升高(P<0.01),结肠组织TLR-2、TLR-4基因及蛋白表达明显增强(P<0.05);与模型组比较,各给药组大鼠血清SOD活性明显升高、MDA含量明显降低(P<0.05,P<0.01),结肠组织TLR-2、TLR-4基因及蛋白表达明显降低(P<0.05,P<0.01)。结论四神丸可能通过改善结肠组织氧自由基的代谢,调控肠上皮免疫系统,达到治疗UC的目的。Objective To observe the effects of Sishen Pills on gene and protein expressions of toll-like receptor 2（TLR-2） and toll-like receptor 4（TLR-4） of colonic tissue in spleen-kidney yang deficiency rats with ulcerative colitis（UC）; To discuss its mechanism of action for spleen-kidney yang deficiency UC. Methods Lavage of senna ＋ intraperitoneal injection of hydrocortisone injection ＋ enema of 2,4,6-trinitrobenzene sulfonic acid/ethanol were used to establish the model of UC of spleen-kidney yang deficiency type. 90 Wister rats were randomly divided into blank group, model group, Salazosulfadimidine group and the Sishen Pills high-, medium-and low-group. Each medication group was intervened by relevant medicine. RT-q PCR was used to detect the expressions of TLR-2 and TLR-4, and Western blot was used to detect the protein expressions of TLR-2 and TLR-4. SOD and MDA in serum were detected. Results Compared with the blank group, SOD activity decreased and MDA content increased in the model group（P〈0.01）; gene and protein expressions of TLR-2 and TLR-4 increased significantly（P〈0.05）. Compared with model group, SOD increased and MDA content decreased in all the medication groups（P〈0.05, P〈0.01）; gene and protein expressions of TLR-2 and TLR-4 decreased（P〈0.05, P〈0.01）. Conclusion Sishen Pills can achieve the treatment of UC by improving the metabolism of oxygen radicals in colonic tissue and regulating the immune system of the intestinal epithelium.

关 键 词：溃疡性结肠炎 四神丸 TOLL样受体2 TOLL样受体4 大鼠 

分 类 号：R285.5[医药卫生—中药学]