猪转化生长因子β受体Ⅱ基因克隆与组织表达特征分析  

Cloning and tissue expression profiles of the TGFBR2 gene in pigs

作　　者：李琦琦 张久峰[1] 刘红林[1] 李齐发[1] 

机构地区：[1]南京农业大学动物科技学院,江苏南京210095

出　　处：《畜牧与兽医》2016年第8期44-49,共6页Animal Husbandry & Veterinary Medicine

基　　金：江苏省江苏省农业科技自主创新资金(SCX(12)2097)

摘　　要：为了研究猪转化生长因子β受体Ⅱ(transforming growth factor beta receptorⅡ,TGFBR2)基因序列特征和组织表达特征,利用克隆测序技术获得二花脸猪TGFBR2基因编码区序列,采用RT-PCR技术分析二花脸猪TGFBR2基因的组织表达特征。结果显示:二花脸猪TGFBR2基因编码区序列长度为1 461 bp,编码蛋白含486个氨基酸残基,含有ec TbetaR2结构域、蛋白激酶结构域等典型的功能域;RT-PCR发现TGFBR2基因在二花脸猪下丘脑、子宫、卵巢和肌肉等12种组织中广泛表达,特别是在卵巢组织中高表达。结果表明:猪TGFBR2基因为进化上相对保守且在卵巢组织中高表达的基因。To investigate the sequence features and tissue specific expression of the TGFBR2 gene in pigs,cloning and sequencing techniques were used to obtain the coding region sequences of TGFBR2 gene in Erhualian pigs. The tissue expression profile of TGFBR2 was detected by RT- PCR. The results showed that the full length of Erhualian pigs＇ TGFBR2 gene coding region was 1461 bp,which coding a protein contains 486 amino acid residues with typical structural domains of ec TbetaR2 and Protein kinase. RT- PCR result showed that TGFBR2 mRNA was extensively expressed in 12 tissues of Erhualian pigs including hypothalamus,uterus,and muscle,whilst ovary tissue appears to have extremely high expression level. In conclusion,TGFBR2 is a revolutionarily conserved gene that highly expressed in the ovary.

关 键 词：二花脸猪 TGFBR2 克隆 组织表达 

分 类 号：S828.8[农业科学—畜牧学]