HPLC法测定联用利福平前后HIV感染者血浆中依非韦伦浓度  

Determination of Efavirenz in HIV Patients' Plasma before and after Co-Administration with Rifampicin by HPLC

作　　者：周波[1] 李燕青[1] 陈谐捷[1] 谭俊[1] 

机构地区：[1]广州市第八人民医院药剂科,广东广州510060

出　　处：《今日药学》2016年第8期541-544,共4页Pharmacy Today

基　　金：广东省省级科技计划项目(2014A020212425);广州市医药卫生科技项目(2014A010030)

摘　　要：目的建立HPLC法测定联用利福平前后HIV感染者血浆中的EFV浓度,为个体化治疗提供依据。方法色谱柱为Agilent Eclipse XDB-C_(18)(4.6 mm×150 mm,5μm),流动相为乙腈∶水(58∶42),流速为0.8 m L/min,检测波长为244 nm,柱温30℃,进样量20μL,测定3例HIV感染者。结果 EFV保留时间为7.8 min,标准曲线方程为y=3.409 5x-0.004 8(r^2=0.999 4),线性范围为0.04~20μg/m L。3例患者使用利福平后EFV浓度均有所下降,平均降幅为0.567μg/m L。结论该方法灵敏、准确可行,可用于测定人血浆中EFV浓度,联用利福平可降低EFV浓度。OBJECTIVE To establish an HPLC method for determining the concentration of Efavirenz in HIV infector＇ s plasma before and after co-administrated with Rifampicin. METHODS The HPLC procedure was performed on the Agilent Eclipse XDB-C18 （4.6 mm× 150 mm, 5 μm） , with the mobile phase composed of acetonitrile-water （ 58：42）, at the flow rate of 0.8 mL/min, the detection wavelength was 244 nm, and column temperature was 30 ℃, the injection volume was 20 μL. RESULTS The EFV＇ s retention time was 7.8 min, the standard curve＇ s equation was y = 3.409 5x- 0. 004 8 （ r2 = 0.999 4） with a linearity range of 0.04- 20 g/mL. 3 patients＇ EFV concentrations were averagely reduced by 0.567 g/mL after co-administrated with Rifampicin. CONCLUSION This determination method is sensitive, accurate, and reliable, and it could be used to detect the concentration of EFV in plasma; after coadministrated with Rifampicin,the concentration of EFV would be declined.

关 键 词：依非韦伦 利福平 相互作用 HPLC 

分 类 号：R97[医药卫生—药品]