基于GC-MS代谢组学的金欣口服液治疗RSV感染的体外研究  

Antiviral Effects of Jinxin Oral Liquid Against Respiratory Syncytial Virus Infection in Vitro Based on Gas Chromatography-Mass Spectrometer Metabolomics

作　　者：孟欣[1] 单进军[1] 谢彤[1] 徐建亚[1] 沈存思[1] 汪受传[1] 

机构地区：[1]南京中医药大学江苏省儿童呼吸疾病(中医药)重点实验室,江苏南京210023

出　　处：《辽宁中医杂志》2016年第8期1585-1589,I0002,共6页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金(81373688;81303295);江苏省研究生培养创新工程(SJZZ15_0120)

摘　　要：目的:通过气相色谱-质谱联用(GC-MS)技术分析Hep-2细胞内源性代谢产物的相对含量变化,阐明金欣口服液体外抗RSV感染的作用机制。方法:RSV感染接种于6孔板上的Hep-2细胞,予金欣口服液治疗,24 h后予以液氮淬灭细胞,先后加入水-甲醇-氯仿(v:v:v,1:8.1:0.9)以便取少量细胞悬浮液行蛋白定量和萃取细胞代谢物,运用GC-MS检测细胞样品内源性代谢物及其相对含量的变化,并分析其可能参与的代谢通路。结果:RSV感染的细胞样本中共测得29种内源性代谢产物,其中谷氨酰胺、γ-氨基丁酸、甘氨酸、柠檬酸、乳酸等为差异代谢产物(P<0.05),主要与谷氨酰胺、甘氨酸、丙酮酸代谢通路有关。结论:金欣口服液体外治疗RSV感染的机制可能与调节RSV感染后引起的代谢紊乱有关。Objective： To detect the relative abundance changes of endogenous metabolites in Hep- 2 cell to explore the mechanism of Jinxin Oral Liquid against respiratory syncytial virus infection in vitro by GC- MS technology. Methods： Hep- 2 cell was infected by RSV in 6- well plates and then Jinxin Oral Liquid was injected. After 24 h of the RSV infection,5 m L liquid nitrogen was poured into the 6- well plates to quench cells,subsequently water- methanol- chloroform（ v： v： v,1： 8. 1： 0. 9） was successively added to prepare the cell sample for protein quantification and extract the endogenous metabolites. Cell samples were detected by GC- MS and possible metabolic pathways concerned were analyzed. Results： Twenty- nine endogenous metabolites of infected cells were detected,among which glutamineγ- aminobutyric acid,glycine,citric acid and lactic acid were statistically significant（ P 〈 0. 05）. The glutamine,glycine and pyruvate metabolic pathways were most concerned. Conclusion： The antiviral effect of Jinxin Oral Liquid against RSV infection may be related to the regulation of metabolic disorder caused by RSV.

关 键 词：GC-MS 金欣口服液 呼吸道合胞病毒 体外研究 

分 类 号：R373.1[医药卫生—病原生物学]