射干止咳有效成分白射干素提取优化  

Optimum Extraction Technology of Anti-cough Composition Dichotomin in Belamcanda Chinensis

作　　者：李欣妍[1] 尤献民[2] 邹桂欣[2] 李国信[2] 

机构地区：[1]辽宁中医药大学,辽宁沈阳110032 [2]辽宁省中医药研究院,辽宁沈阳110034

出　　处：《辽宁中医杂志》2016年第8期1699-1701,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81273927);国家"十二五"重大新药创制项目(2012zx09303-017);国家临床重点专科建设项目经费资助项目;国家中医药管理局临床中药学重点学科;辽宁中医药大学杏林学者青蓝工程

摘　　要：目的:优化射干抗炎止咳有效成分白射干素的提取工艺。方法:以白射干素含量为指标,采用HPLC-DAD检测,对药材提取过程中提取溶剂、溶剂倍数以及提取时间3个影响因素进行L9(34)正交试验分析,设计优选出射干中白射干素的提取工艺。结果:白射干素最佳提取工艺为加70%乙腈8倍量、8倍量提取2次,每次提取1 h。结论:优选出的提取工艺稳定可行,白射干素提取率高,适于射干药材中白射干素的提取。Objective： To optimize the extract process of dichotomin in Belamcanda chinensis. Methods： HPLC- DAD detector was used for determining dichotomin. An orthogonal test design with different levels of content ration of solvents,extraction time and volume of the solvent was implemented to determine the optimized extract condition. Results： The optimized parameters of best extractions were established： 70% acetonitrile,8 times,extraction twice,for 1 h. Conclusion： The optimum extraction process is reliable and the extraction ratio of active components is high.

关 键 词：射干 白射干素 提取工艺 正交实验 

分 类 号：R284.2[医药卫生—中药学]