独活香豆素对Aβ所致神经元损伤的神经保护作用研究  

Neuroprotective Effects of Total Coumarins in Angelica Pubescens against Aβ-Induced Neurons Damage

作　　者：闫宇辉[1] 李少恒[1] 孔亮[1] 教亚男 姚璎珈 陶震宇 宋捷[1] 杨静娴[1] 

机构地区：[1]辽宁中医药大学药学院药理学教研室,辽宁大连116600

出　　处：《辽宁中医杂志》2016年第8期1714-1717,I0003,共5页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81173580)

摘　　要：目的:探究独活香豆素(TCA)对Aβ所致的小鼠海马神经元损伤的神经保护作用及其作用机理。方法:从胎鼠大脑海马中分离出神经元细胞,培养9 d待神经元成熟后将细胞分为正常组(control)、模型组(30 mmol/L Aβ孵育24 h)和给药组(经Aβ处理后给予不同浓度TCA处理的细胞)。MTT法和LDH法检测各组海马神经元的活性,RTPCR法检测环磷酸腺苷反应元件结合蛋白(CREB)和脑源性神经营养因子(BDNF)mRNA表达的变化,免疫组织化学染色法检测突触素-1(synapsin^(-1))、磷酸化环磷酸腺苷反应元件结合蛋白(p-CREB)和BDNF蛋白表达的变化。结果:孵育Aβ的海马神经元显现较强神经毒性。与模型组比较,TCA组明显增强神经细胞的存活能力,增加synapsin^(-1)的表达量,提高BDNF mRNA的表达,增强p-CREB和BDNF蛋白的表达。结论:TCA对Aβ所致的海马神经元损伤具有保护作用,此神经保护作用可能是与促进CREB的磷酸化使p-CREB表达增多,并刺激神经营养因子BDNF的生成增多有关。Objective： To investigate the neuroprotective effects and mechanism of total coumarins in Angelica Pubescens（ TCA）against Aβ- induced neurons damage. Methods： Primary neurons were isolated and purified from cerebral cortex of suckling mice.And then neurons were divided into three groups： control,Aβ and TCA groups. MTT and LDH were used to observe cell viability of neuron. CREB and BDNF mRNA expressions were detected by RT- PCR and immunocytochemical staining was performed to determine the expressions of SYN- 1,p- CREB and BDNF. Results： Compared with the control group,TCA group significantly increased cell viability,promoted the expression of synapsin 1 and increased the expressions of p- CREB and BDNF. Conclusion：TCA possesses the neuroprotective effects on Aβ damaging neuron,which may be associated with the enhancement of CREB phosphprylation.

关 键 词：独活香豆素 阿尔茨海默病 海马神经元 磷酸化环磷酸腺苷反应元件结合蛋白 脑源性神经营养因子 

分 类 号：R285.5[医药卫生—中药学]