UPLC-MS/MS法测定大鼠灌服稳心颗粒后血浆中3个皂苷成分及其药动学研究  

UPLC-MS/MS Simultaneous Determination and Pharmacokinetics of Three Saponins in Rats Splasma after Taking Wenxin Granules

作　　者：张家伟[1,2] 王晓明[1] 潘桂湘[1] 李婷婷[1,2] 朱彦[1,2] 

机构地区：[1]天津中医药大学天津市现代中药重点实验室-省部共建国家重点实验室(培育),天津300193 [2]天津国际生物医药联合研究院,天津300457

出　　处：《辽宁中医杂志》2016年第8期1717-1720,共4页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(81173525;813031827);国际科技合作专项(2013DFA31620)

摘　　要：目的：采用UPLC-MS/MS法测定大鼠灌服稳心颗粒后血浆中3个皂苷成分的浓度,并研究其药动学特征。方法：血浆样品以甲醇-乙腈（1：1）沉淀蛋白,采用CORTECS-C18色谱柱（2.1 mm×50 mm,1.6μm）,以0.1%甲酸水（A）-乙腈（含0.1%甲酸）（B）为流动相进行梯度洗脱,于ESI负离子模式下检测。结果：人参皂苷Rd和Rg1在0.5~20 ng/m L,人参皂苷Rb1在1~40 ng/m L范围内线性关系良好（r大于0.99）,3个成分的提取回收率为人参皂苷Rd105.2%~120.7%,人参皂苷Rg1 101.9%~123.9%,人参皂苷Rb1 92.8%~116.9%。结论：该方法简便、准确、灵敏,可用于稳心颗粒药动学研究。Objective： To determine three saponins in rats plasma by UPLC- MS/MS method and study their pharmacokinetics. Methods： Sample preparation was performed by a protein precipition with methanol and acetonitrile（ 1： 1）. The analysis was in the flow rate of 0. 5 m L/min,the column temperature of 40℃ and the injection volume of 5 μL by gradient elution on a CORTECS- C18column（ 2. 1 mm × 50 mm,1. 6 μm） and the mobile phase was 0. 1% formic acid in water（ A）- 0. 1% formic acid of acetonitrile（ B）. Results： All calibration curves had good linearity（ r 〉 0. 99） over the concentration ranges of 0. 5- 20 ng·m L-1for ginsenoside Rd and ginsenoside Rg1 1- 40 ng·m L-1for ginsenoside Rb1. The method recoveries of three saponins were 105.2%- 120. 7% for ginsenoside Rd,101. 9%- 123. 9% for ginsenoside Rg1,92. 8%- 116. 9% for ginsenoside Rb1. Conclusion：The above mentioned method is simple,accurate and sensitive for determination of saponins in rats plasma. and has been successfully applied to the study of pharmacokineties of wenxin Granules.

关 键 词：液质联用 稳心颗粒 皂苷 血药浓度 药动学 

分 类 号：R965[医药卫生—药理学]