玉米磷酸烯醇式丙酮酸羧化酶(PEPC)基因的克隆及序列分析  

Cloning and Bioinformatics Analysis of Phosphoenolpyruvate Carboxylase( PEPC) in Maize

作　　者：王重[1,2] 樊哲儒[1,2] 张跃强[1,2] 李剑峰[1,2] 高新[1,2] 

机构地区：[1]新疆农业科学院核技术生物技术研究所,新疆乌鲁木齐830091 [2]农业部荒漠绿洲作物生理生态与耕作重点实验室,新疆乌鲁木齐830091

出　　处：《安徽农业科学》2016年第20期114-117,共4页Journal of Anhui Agricultural Sciences

基　　金：新疆农业科学院青年基金项目(xjnkq-2013025)

摘　　要：[目的]获得玉米的PEPC基因,并对其生物信息学进行分析。[方法]使用RT-PCR方法由玉米中获得PEPC全长c DNA,构建克隆载体后进行测序,并对其序列进行生物信息学分析。[结果]获得的玉米PEPC基因CDS全长2 913 bp,编码的多肽链包含970个氨基酸,为疏水性氨基酸,由α-螺旋（61.44%）、无规则卷曲（34.43%）和延伸链（4.12%）组成,定位于细胞质,不存在跨膜结构域,其175970位氨基酸组成了磷酸烯醇式丙酮酸羧化酶的功能结构域,在173184、597609位具有2个磷酸烯醇式丙酮酸羧化酶活性位点。[结论]该研究克隆了玉米C4型丙酮酸磷酸双激酶（PPDK）基因,它所编码的氨基酸序列具备PPDK蛋白的保守序列和催化活性中心区域。[Objective]To clone the phosphoenolpyruvate carboxylase（ PEPC） gene obtained from Zea mays and analyze it by bioinformatics.[Method]Primarily,the c DNA clone was constructed by RT-PCR amplified with the gene specific primers,then positive clones were sequenced and analyzed by bioinformatics. [Result]The electrophoresis showed that the coding sequence（ CDS） of PEPC gene in Zea mays was2 913 bp and the polypeptide chains coded by PEPC included 926 amino acids,which were hydrophobic amino acids consists of-helix（ 61. 44%）,random coil（ 34. 43%） and extended strand（ 4. 12%）,with located in the cytoplasm. There was no transmembrane domain.The 175- 970 amino acid composition the functional domains of phosphoenolpyruvate carboxylase,in 173- 184; 597- 609 bits had two phosphoenolpyruvate carboxylase active site with pyruvate orthophosphate dikinase basing on amino acids sequences comparison. [Conclusion]The PEPC gene in Zea mays has been obtained and the amino acids sequence coded by the gene was conserved and contained active catalyst central site of PEPC.

关 键 词：C4植物 光合作用效率 PEPC 生物信息学分析 

分 类 号：S188[农业科学—农业基础科学]