一种用于桑RAD-Seq高通量测序的基因组DNA提取方法  

A High Throughput Sequencing DNA Extraction Method for Morus RAD- Seq

作　　者：刘玲[1] 柯皓天 吕银 陈仁芳[3] 

机构地区：[1]四川省丝绸科学研究院,四川成都610031 [2]四川省丝绸工程技术研究中心,四川成都610031 [3]西南大学生物技术学院,重庆400716

出　　处：《安徽农业科学》2016年第21期136-137,共2页Journal of Anhui Agricultural Sciences

摘　　要：[目的]筛选一种能够得到高质量桑树基因组DNA的方法。[方法]采用天根试剂盒法(Plant Genomic DNA Kit离心柱型),并稍加改进,从11个桑种的新鲜嫩叶中提取DNA。[结果]该方法简单快速,不需要复杂仪器,且能有效地除去桑叶中的多糖、多酚、蛋白质等杂质,得到的DNA能够满足RAD-Seq高通量的测序要求。[结论]建立了一种用于桑RAD-Seq高通量测序的基因组DNA提取方法。[Objective] The aim was to screen out a method to obtain high quality DNA from mulberry genome. [Method] By using improved Plant Genomic DNA Kit centrifugal column method,DNA was extracted from 11 species of fresh mulberry leaves.[Result] The method is simple,rapid,and can effectively remove polysaccharides,polyphenols,protein and other impurities in the mulberry leaves,the DNA is able to meet the RAD-Seq high-throughput sequencing requirements.[Conclusion]A DNA extraction method for Morus RAD-Seq sequencing was established.

关 键 词：桑树 RAD-Seq 基因组DNA提取 

分 类 号：S188[农业科学—农业基础科学]