针康法对局灶性脑梗死大鼠神经功能缺损及梗死灶周边区RhoA、ROCK-Ⅱ蛋白表达的影响  

Effect of Cluster Needling of Scalp Acupuncture Combined with Rehabilitation on Neurological Function and Expression of Rho A and ROCK-Ⅱ in the Cortical Peri-infarct Area of Rats after Focal Permanent Cerebral Ischemia

作　　者：唐强[1] 叶涛[2] 朱路文[1] 张金朋[1] 姜云飞[2] 吴孝军[2] 

机构地区：[1]黑龙江中医药大学附属第二医院,黑龙江哈尔滨150001 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《针灸临床杂志》2016年第8期73-77,共5页Journal of Clinical Acupuncture and Moxibustion

基　　金：国家自然科学基金项目;编号:81473762

摘　　要：目的:探讨针康法对局灶性脑梗死大鼠神经功能缺损及梗死灶周边区Rho A、ROCK-II蛋白表达的影响。方法:将90只清洁级SD雄性大鼠随机分为5组,即假手术组、模型组、针刺组、康复组及针康组,各组于3天、7天、14天时间点再分成3个亚组(n=6)。采用改良的Zea Longa线栓法制备局灶性脑梗死大鼠模型。假手术组与模型组大鼠不予任何治疗,针刺组给予头穴丛刺治疗,康复组给予运动康复训练,针康组给予头穴丛刺结合运动康复训练。术后3天、7天、14天采用改良大鼠神经功能缺损评分(m NSS)评估各组大鼠神经功能,免疫组化法检测大鼠梗死灶周边区Rho A、ROCK-Ⅱ蛋白的表达。结果:术后7天、14天,与模型组比较,各干预组大鼠神经功能缺损评分均明显降低(P<0.05);与针刺组、康复组比较,针康组大鼠评分有所降低(P<0.05)。术后各时间点,与模型组比较,各干预组梗死灶周边区Rho A、ROCK-Ⅱ阳性细胞数均明显减少(P<0.05);与针刺组、康复组比较,针康组大鼠梗死灶周边区Rho A、ROCK-Ⅱ阳性细胞数目有所减少(P<0.05)。结论:头穴丛刺疗法、运动康复训练、针康法均能有效改善局灶性脑梗死大鼠神经功能缺损,下调梗死灶周边区Rho A、ROCK-Ⅱ蛋白表达,且针康法优于单纯头穴丛刺或运动康复训练。Objective： To investigate the effect of cluster needling of scalp acupuncture combined with rehabilitation on neurological function and expression of RhoA and ROCK- Ⅱ in the cortical peri- infarct area of rats after focal permanent cerebral ischemia. Methods： 90 male SD rats were randomly divided into five groups,namely,sham control group（ SC）,model group（ M）,scalp acupuncture group（ SA）,rehabilitation training group（ RT） and acupuncture- rehabilitation therapy group（ ART）. Each group was subdivided into 3 subgroups by 3 d,7 d,14 d（ n = 6）. The rats in M,SA,RT,ART groups were made into the models with focal permanent cerebral ischemia by modified Zea Longa method. Rats in SC group and M group were without any intervention. SA group was treated by cluster needling of scalp acupuncture therapy; RT group was treated by rehabilitation training; ART group received acupuncture- rehabilitation therapy. Neurological function was evaluated by modified neurological severity score（ m NSS）,and the expressions of RhoA and ROCK-Ⅱ in the cortical peri- infarct area of rats were observed by immunohistochemistry staining in rats in each group after 3 d,7 d,14 d of surgery. Results： After 7 d,14 d of surgery,compared with SA group and RT group,the m NSS was reduced in ART group,and the difference was statistically significant（ P〈0. 05）. After 3 d,7 d,14 d of surgery,compared with SA group and RT group,the number of positive cells of RhoA and ROCK-Ⅱ was decreased in ART group,and the difference was statistically significant（ P〈0. 05）. Conclusion： Acupuncture- rehabilitation therapy can improve the neurological function defect in rats with focal permanent cerebral ischemia,and its therapeutic mechanism may be related to down- regulating the expressions of RhoA and ROCK-Ⅱ in the cortical peri- infarct area.

关 键 词：针康法 脑梗死 神经功能缺损 Ras基因同源物A Rho激酶2 

分 类 号：R246.6[医药卫生—针灸推拿学]