响应面优化矮地茶多糖提取工艺研究  

Optimization of Extraction Technology of Aidicha(Ardisia japonica)Polysaccharides by Response Surface Methodology

作　　者：黄懿[1] 王秀梅[1] 潘涛[1] 田霞[1] 肖作奇 

机构地区：[1]湖南省妇幼保健院,湖南长沙410008

出　　处：《中医药导报》2016年第18期57-60,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省中医药管理局科研项目(201589)

摘　　要：目的:优化矮地茶多糖提取工艺。方法:采用苯酚-硫酸法建立矮地茶中糖含量测定方法,同时以糖含量为指标,采用响应面法对矮地茶多糖提取温度、提取时间、料液比3个影响因素进行工艺优化。结果:建立了矮地茶多糖提取模型,回归模型显著;提取优化条件为:提取温度90℃,提取时间1.8 h,料液比1∶32。结论:该提取工艺合理,矮地茶多糖的提取率高。Objective： To optimize the extraction technology of Ardisia japonica Polysaccharides （AJP）. Meth- ods： Phenol-sulfuric methods were used for the detection of the content of polysaccharides from Ardisia japonica. Extraction temperature, extraction duration and solid- liquid ratio were investigated by Response surface method- ology using the yield of AJP as index. Results： The extraction process of AJP was build, and the model is re- markable. The best conditions gained： the extraction temperature was 90 ℃, the distilled time was 1.8 h, and the solid- liquid ratio was 1：32. Conclusion： The extraction technology is reasonable, and AJP has high extraction rate.

关 键 词：矮地茶 多糖 响应面法 提取工艺 苯酚-硫酸 

分 类 号：R284.2[医药卫生—中药学]