山东道地药材丹参脂溶性成分指纹图谱及多指标成分同时定量研究  

Study on Fingerprint and Simultaneous Determination of Multi-components of Lipid-soluble Components of Genuine Salvia miltiorrhiza Bunge in Shandong Province

作　　者：窦锦明[1] 王振华[1] 徐楠[2] 荆汉卫 

机构地区：[1]潍坊市中医院,山东潍坊261041 [2]山东省中医药研究院,山东济南250014 [3]潍坊市食品药品检验研究院,山东潍坊261041

出　　处：《山东中医杂志》2016年第8期736-738,753,共4页Shandong Journal of Traditional Chinese Medicine

基　　金：潍坊市中医药科研立项项目(2015年第一类编号006号)

摘　　要：目的：建立山东道地药材丹参脂溶性成分指纹图谱，并对其三种脂溶性成分同时进行含量测定。方法：Sciences—InertSustainC．8色谱柱；乙腈-0．02％磷酸溶液梯度洗脱；流速1．0mL／min；温度30℃。结果：10批次丹参指纹图谱标定20个共有峰，相似度均≥0．940；丹参酮Ⅰ、丹参酮ⅡA、隐丹参酮在0．11～2．2、0．0521．04和0．043～0．086μg之间呈良好的线性关系。结论：所建立的方法可靠、结果稳定，为丹参药材的质量评价提供参考依据。Objective：To establish the fingerprint of lipid-soluble components of genuine salvia miltiorrhiza bunge in Shandong and to perform simultaneous determination of three components in it. Methods：The anal- ysis were performed on Sciences-InertSustain Cls column,with acetonitrile-0.02% phosphoric acid solution as the eluent. The flow rate was 1.0 mL/min and the temperature was 30 ℃. Results：Twenty common peaks were identified form the fingerprints of 10 batches of salvia miltiorrhiza bunge and the similarities were not less than 0.940. The linear ranges of tanshinone Ⅰ ,tanshinone Ⅱ A and cryptotanshinone were 0.11-2.2 μ, 0.052-1.04 μg and 0.043--0.086. Conclusion：The established method is simple and the result was accurate. They can provide reference for quality control of salvia mihiorrhiza bunge.

关 键 词：丹参 脂溶性成分 指纹图谱 多指标成分 定量分析 

分 类 号：R284.2[医药卫生—中药学]