Genome-Wide Sequencing of 41 Rice （Oryza sativa L.） Mutated Lines Reveals Diverse Mutations Induced by Fast-Neutron Irradiation  

作　　者：Li, Guotian Chern, Mawsheng Jain, Rashmi Martin, Joel A. Schackwitz, Wendy S. Jiang, Liangrong Vega-Sanchez, Miguel E. Lipzen, Anna M. Barry, Kerrie W. Schmutz, Jeremy Ronald, Pamela C. 

机构地区：[1]Univ Calif Davis, Dept Plant Pathol, Davis, CA 95616 USA [2]Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA [3]Lawrence Berkeley Natl Lab, Feedstocks Div, Joint BioEnergy Inst, Berkeley, CA 94720 USA [4]US DOE, Joint Genome Inst, Walnut Creek, CA 94598 USA [5]Xiamen Univ, Sch Life Sci, Xiamen 361102, Peoples R China [6]HudsonAlpha Inst Biotechnol, Huntsville, AL 35806 USA [7]Monsanto Co, Chesterfield Village Campus, Chesterfield, MO 63017 USA

出　　处：《Molecular Plant》2016年第7期1078-1081,共4页分子植物（英文版）

摘　　要：Fast-neutron （FN） irradiation has been used to create mutagen- ized collections of many plant species （Bolon et al., 2014）, FN- induced mutagenesis has clear advantages： it is an efficient means of saturating the genome, and it does not involve time- consuming plant transformation or tissue culture. In rice, most mutant collections, although highly valuable, were generated us- ing either T-DNA insertion or transposon tagging approaches that often induce mutations unlinked to the insertion and complicating analysis （Wang et al., 2013）. Another disadvantage of some of these collections is that they were created in rice varieties that are photoperiod-sensitive and often have long generation times.Fast-neutron （FN） irradiation has been used to create mutagen- ized collections of many plant species （Bolon et al., 2014）, FN- induced mutagenesis has clear advantages： it is an efficient means of saturating the genome, and it does not involve time- consuming plant transformation or tissue culture. In rice, most mutant collections, although highly valuable, were generated us- ing either T-DNA insertion or transposon tagging approaches that often induce mutations unlinked to the insertion and complicating analysis （Wang et al., 2013）. Another disadvantage of some of these collections is that they were created in rice varieties that are photoperiod-sensitive and often have long generation times.

分 类 号：S511[农业科学—作物学] S857.144