大孔树脂分离纯化西洋参叶总皂苷的工艺研究  

Purification of Total Saponins of Leaves of Panax quinquefolius by Macroreticular Resin

作　　者：郭婷婷[1] 王兆华[2] 张大军[2] 

机构地区：[1]长春中医药大学附属医院,吉林长春130021 [2]吉林省中医药科学院,吉林长春130012

出　　处：《中国现代中药》2016年第9期1196-1200,共5页Modern Chinese Medicine

基　　金：国家科技重大专项(2013ZX09103002-022);吉林省科技发展计划专项(20140311014YY)

摘　　要：目的:探索以D101型大孔吸附树脂分离纯化西洋参叶总皂苷的工艺条件及参数。方法:以总皂苷及人参皂苷Rb3的吸附率和解吸附率为指标,对大孔树脂分离纯化西洋参叶总皂苷的工艺条件进行考察,并对其吸附动力学曲线和动态解析曲线进行探索。结果:药材与湿树脂量比为3∶10,上柱流速为1.5 m L·min-1,重复上样两次,分别用3倍体积的水、3倍体积70%乙醇洗脱,收集70%乙醇洗脱液,减压,浓缩,干燥,即得总皂苷提取物。提取物中总皂苷含量>75%,人参皂苷Rb3含量>10%,总皂苷提取物得率约为14%。结论:该工艺简便,重复性好,可用于西洋参叶中总皂苷的提取纯化。Objective： The study aims at purification of total saponins of leaves of Panax quinquefloius by D-101 macroreticular resin. Methods： Using the adsorption and desorption rates of total saponins and ginsenside Rb3 as parameters, the purification technological conditions of total saponins of the leaves of P. quinquefloius by macroreticular resin were experimentally investigated. The curves of adsorption kinetics and dynamic adsorption kinetics of macroreticular resin were exploited. Results： The rate of medicinal materials and the wet resin content was 3：10. The flow rate of the macro-reticular resin column was 1.5 mL·min^-1. The experiments were repeated twice. The column was eluted by water of three times volume and ethanol of three times volume. After then ethanol elution was collected. The ethanol elution was concentrated, dried, and then the total saponins extract of the leaves of P. quinquefloius was obtained. The contents of total saponins in extract was more than 75%. The contents of ginsenside Rb3 was more than 10%. The yield of total saponins extract was 14%. Conclusion： This method is simple, repeatable, available for extract and purification of total saponins of the leaves of P. quinquefloius.

关 键 词：西洋参叶 大孔吸附树脂 总皂苷 人参皂苷RB3 工艺参数 

分 类 号：R284.1[医药卫生—中药学]