果蝇新品系开发与种质资源保存  

The Standard of Drosophila Strain Development and Preservation

作　　者：王霞[1,2,3] 乔欢欢[1] 潘丽霞[1] 任兴杰[1] 孙锦[1] 徐荣刚[1] 刘鲁萍[1] 贺争鸣 倪建泉 

机构地区：[1]清华大学医学院,北京100084 [2]中国食品药品检定研究所,北京100050 [3]北京大学生命科学学院,北京100871

出　　处：《实验动物科学》2016年第3期4-12,共9页Laboratory Animal Science

基　　金：国家科技支撑计划(No.2015BAI09B03)

摘　　要：黑腹果蝇(Drosophila melanogaster)是当今生物医学研究领域最重要的模式生物之一。良好的饲养、高效的转基因构建和规范化的品系保存是顺利开展果蝇实验的三大基石。清华大学果蝇中心作为国内最大的果蝇资源平台,积累了丰富的果蝇操作和维护经验。本文综合介绍了清华大学果蝇中心在日常饲养、品系开发和种质保存等方面建立的标准化操作体系,旨在为广大科研同仁提供经验参考,加快我国在以果蝇为模式生物研究生物医学重大问题等领域的研究进程。Drosophila melanogaster is one of the most important model organisms in biological and medical research.It is understood that the three pillars of successful Drosophila experiments are： proper culture condition,efficient transgenic technology,and standardized strain preservation. As the biggest fly resource platform in China,Tsinghua Fly Center（ THFC） is experienced in fly genetics and fly preservation. This article summarizes the standard methods of fly culture,new strain production and strain preservation developed by THFC,aiming to provide information for the Chinese Drosophila research community,and to expedite important biological and medical researches that use Drosophila as model organism.

关 键 词：果蝇 日常饲养 品系开发 种质保存 标准化 

分 类 号：Q95-331[生物学—动物学]