破壁灵芝孢子粉胶囊毒性的实验研究  

Experimental Study on the Toxicity of Broken Ganoderma Lucidum Spore Powder Capsule

作　　者：邱诗宝 周明 李启富 褚芳 

机构地区：[1]江西省职业病防治研究院,南昌330006

出　　处：《实验动物科学》2016年第3期27-31,共5页Laboratory Animal Science

摘　　要：目的观察破壁灵芝孢子粉胶囊毒性。方法 (1)急性毒性试验:选用昆明种小鼠20只,雌雄各半,实验前禁食16 h,24 h内灌胃3次,剂量为15 g/kg BW。灌胃后,连续观察7 d,记录中毒表现及死亡情况。(2)30 d喂养试验:断乳大鼠,体质量60 g左右,80只,雌雄各半。随机分为4组,即对照组和高、中、低剂量实验组(1.5、0.75、0.375 g/kg BW),各剂量组在基础粉料中分别掺入1.5%、0.75%、0.375%的受试物,单笼喂养,自由饮食,记录大鼠进食量、体质量,连续喂养并观察30 d。观察动物的一般表现、体质量、食物利用率,血常规及生化指标:白细胞计数及其分类、红细胞计数、血红蛋白、谷草转氨酶、谷丙转氨酶、尿素氮、肌酐、胆固醇、甘油三酯、血糖、总蛋白、白蛋白。结果各剂量组动物体质量、食物利用率与对照组比较差异无显著性(P>0.05)。其血液指标:白细胞计数及其分类、红细胞计数、血红蛋白;生化指标:谷丙转氨酶、谷草转氨酶、尿素氮、肌酐、胆固醇、甘油三脂、血糖、总蛋白、白蛋白均在正常值范围;病理学检查未见有意义的病理变化。该受试物对两种性别小鼠的急性毒性LD50均大于15 g/kg BW。结论破壁灵芝孢子粉胶囊属无毒级。Objective To observe the toxicity of broken ganoderma lucidum spore powder capsule. Method（ 1）Acute toxicity test： select 20 Kunming mice in total by male and female in half,fasted for 16 h before the experiment,intragastric administration for three times in 24 h with the dose of 15 g / kg BW. After intragastric administration,continuous observation for 7 d and record poisoning manifestations and death case.（ 2） 30 d feeding test： select 80 weaning rats with the weight about 60 g by male and female in half. The 80 rats were randomly divided into four groups,control group and high,and,low experimental group（ 1. 5,0. 75,0. 375 g / kg BW）,each dose group was incorporated into the subject matter with 1. 5%,0. 75%,0. 375% at the basis of powder subjects,single cage feeding and free diet for 30 days continuously,then observing and recording the feed intake and body weight of the rats. The general performance of the rats,weight,food utilization rate,blood routine and biochemical indexes such as white blood cell count and classification, the red blood cell count, hemoglobin, aspartate aminotransferase,alanine aminotransferase, blood urea nitrogen, creatinine, cholesterol, triglycerides, blood glucose,total protein,albumin were observed. Result There was no significant difference in body weight,food utilization rate between control group and each dose group（ P 〉 0. 05）. The blood index： white blood cell count,classification,red blood cell count,hemoglobin and biochemical indexes： alanine aminotransferase,aspartate aminotransferase,blood urea nitrogen,creatinine,cholesterol,triglyceride,blood glucose,total protein and albumin were within the normal range. The acute toxicity of subject matter LD50 were greater than 15 g / kg BW for the two sexes of mice. Conclusion The broken ganoderma lucidum spore powder capsule is non- toxic.

关 键 词：破壁灵芝孢子粉胶囊 毒性试验 

分 类 号：R927.1[医药卫生—药学]