Influence of amphotericin B on liquid crystal state of the Cholesterol/Dipalmitoylphosphatidylcholine monolayer in the presence of different metal cations  

作　　者：王娟 史瑞新 孙润广 郝长春 李俊花 逯晓龙 

机构地区：[1]Laboratory of Biophysics and Biomedicine,College of Physics and Information Technology,Shaanxi Normal University [2]School and Hospital of Stomatology,Jilin University

出　　处：《Chinese Physics B》2016年第9期42-48,共7页中国物理B（英文版）

基　　金：Project supported by the National Natural Science Foundation of China(Grant Nos.21402114 and 11544009);the Natural Science Basic Research Plan in Shaanxi Province of China(Grant No.2016JM2010);the Fundamental Research Funds for the Central Universities of China(Grant No.GK201603026)

摘　　要：Amphotericin B is a very effective antifungal drug,but it has an adverse reaction to the membrane of mammals＇ cells.The interaction between Am B and cholesterol（Chol） causes the formation of pores on the membrane to destroy its integrity.In particular,Am B has a significant effect on the permeability of membrane for K~＋ions.It has been reported that Na＋ions and Ca^（2＋）ions may have some influence on the interaction between amphotericin B and lipid molecules.In this work,the effects of these metal cations on the physical state and intermolecular interaction of the Cholesterol/Dipalmitoylphosphatidylcholine（Chol/DPPC） monolayer with and without Am B have been investigated.The addition of Am B induces the change of physical state of the lipid monolayer from liquid-gel phase to liquid phase.Different metal cations could influence the phase transition of the Am B-lipid monolayer.The K~＋ions and Ca2＋ions make the obvious phase transition disappear.However,the presence of Na＋ions has little influence on the phase transition of the Am B-lipid monolayer.The addition of Am B and the presence of different metal cations weaken the attractive force on the monolayers.After addition of Am B,the force between the molecules is the strongest in the environment of K＋ions,thus is the weakest in the environment of Ca^（2＋）ions,which may be due to the distribution of these metal cations inside and outside of cells.A large number of K＋ions distribute inside of the cells,thus most of Na＋and Ca^（2＋）ions exist out of the cells.Hence,it may be possible that when Am B molecules are out of the cells,the reaction between the drug and lipid molecules is weaker than that inside the cells.These results may have a great reference value for further studying the toxicity mechanism of Am B and the influence of metal cations on the membrane.Amphotericin B is a very effective antifungal drug,but it has an adverse reaction to the membrane of mammals＇ cells.The interaction between Am B and cholesterol（Chol） causes the formation of pores on the membrane to destroy its integrity.In particular,Am B has a significant effect on the permeability of membrane for K~＋ions.It has been reported that Na＋ions and Ca^（2＋）ions may have some influence on the interaction between amphotericin B and lipid molecules.In this work,the effects of these metal cations on the physical state and intermolecular interaction of the Cholesterol/Dipalmitoylphosphatidylcholine（Chol/DPPC） monolayer with and without Am B have been investigated.The addition of Am B induces the change of physical state of the lipid monolayer from liquid-gel phase to liquid phase.Different metal cations could influence the phase transition of the Am B-lipid monolayer.The K~＋ions and Ca2＋ions make the obvious phase transition disappear.However,the presence of Na＋ions has little influence on the phase transition of the Am B-lipid monolayer.The addition of Am B and the presence of different metal cations weaken the attractive force on the monolayers.After addition of Am B,the force between the molecules is the strongest in the environment of K＋ions,thus is the weakest in the environment of Ca^（2＋）ions,which may be due to the distribution of these metal cations inside and outside of cells.A large number of K＋ions distribute inside of the cells,thus most of Na＋and Ca^（2＋）ions exist out of the cells.Hence,it may be possible that when Am B molecules are out of the cells,the reaction between the drug and lipid molecules is weaker than that inside the cells.These results may have a great reference value for further studying the toxicity mechanism of Am B and the influence of metal cations on the membrane.

关 键 词：Langmuir monolayer liquid state potassium ion intermolecular interaction 

分 类 号：R96[医药卫生—药理学]