土壤中一株抗生素和重金属交叉抗性菌的筛选及抗性研究  

Screening on resistance strains of antibiotic and heavy metal and its resistance research in soil

作　　者：杨芬[1,2] 巢波[2] 杨统一[2] 刘洋波[2] 唐玉斌[2] 

机构地区：[1]江苏科技大学生物技术学院,江苏镇江212018 [2]江苏科技大学环境与化学工程学院,江苏镇江212018

出　　处：《江苏科技大学学报（自然科学版）》2016年第3期303-308,共6页Journal of Jiangsu University of Science and Technology:Natural Science Edition

基　　金：国家自然科学基金资助项目(31400448);中国博士后科学基金资助项目(2014M551605);江苏省博士后科研资助计划项目(1401084B);本科创新计划项目

摘　　要：通过对土壤中分离得到的一株头孢霉素的抗生素抗性菌C-1进行生理生化特性、分子生物学鉴定、不同Zn2+浓度下细菌的生长曲线及抗生素和Zn2+的交叉耐药性研究,探索重金属Zn2+对土壤抗生素抗性菌抗性的影响.结果表明:抗头孢霉素细菌C-1为假单胞菌属(Pseudomonas),生长繁殖易受Zn2+抑制,同时对Zn2+有一定抗性.对7种抗生素进行药敏试验发现,菌株C-1仅对链霉素和磺胺噻唑敏感,Zn2+与链霉素或磺胺噻唑共存时表现出协同杀菌性;菌株C-1具有多重耐药性,对庆大霉素和Zn2+、新霉素和Zn2+有交叉抗性;菌株C-1生长的最低Zn2+抑菌浓度为50 mg/L.In order to explore the resistance influence of heavy metal Zn^2＋ to antibiotic-resistant bacteria in soil,a cephalosporin-resistant strain,designated C- 1,was isolated from soil,its physiological and biochemical characteristics,identification of molecular biology,the growth curve of bacteria in different concentrations of Zn^2＋ and cross-resistance of antibiotics / Zn^2＋ were studied. The results showed that the strain C- 1 belongs to Pseudomonas; it has resistance to Zn^2＋ ,though the growth of it was inhibited by Zn^2＋ in some degree; the susceptibility testing found that this strain only sensitive to streptomycin and sulfathiazole,Zn^2＋ coexisted with streptomycin or sulfathiazole exhibited synergistic bactericidal effect to the strain; the C- 1 has multiple drug resistance,meanwhile having cross resistance to gentamicin / Zn^2＋ and neomycin / Zn^2＋ ; the minimum inhibitory concentration of C- 1 to Zn^2＋ reached 50 mg / L.

关 键 词：抗生素抗性菌 重金属Zn2+ 土壤 头孢霉素 

分 类 号：Q939.96[生物学—微生物学]