甜菜碱醛脱氢酶(BADH)基因转化大豆的研究  

Transformation of Soybean with Betaine Aldehyde Dehydrogenase(BADH)Gene

作　　者：尚丽霞[1] 蔡勤安[1] 于志晶[1] 杨向东[1] 孟凡钢[2] 马瑞[1] 

机构地区：[1]吉林省农业科学院农业生物技术研究所,长春130033 [2]吉林省农业科学院大豆研究所,长春130033

出　　处：《东北农业科学》2016年第2期35-38,共4页Journal of Northeast Agricultural Sciences

基　　金：吉林省科技发展计划项目(20130206005NY);农业部转基因专项(2014ZX0800403B-002)

摘　　要：本研究利用农杆菌介导法将甜菜碱醛脱氢酶(BADH)基因导入大豆,以提高大豆的耐盐性。对132棵转化植株进行了PCR检测,获得64棵阳性植株,阳性率达到48%。对PCR阳性植株进一步进行Southern杂交分析,证明BADH基因已经整合到大豆基因组中。Betaine aldehyde dehydrogenase （BADH） gene was transferred into soybean by using agrobacterium in order to enhance the salt tolerance of soybean. PCR analysis for 132 transformed soybean plants showed that 64 plants were positive and the ratio of positive plant was 48%. Southern blot indicated that BADH gene had been integrated into the genome of soybean.

关 键 词：大豆 BADH基因 遗传转化 

分 类 号：S565.103.53[农业科学—作物学]