黑龙江省和吉林省谷子品种遗传多样性分析  

Analysis of Genetic Diversity of Foxtail Millet of Heilongjiang Province and Jilin Province

作　　者：沈琰 王颖[1,2] 张东杰[1] 杨义杰[1] 赵雅楠[1] 张桂芳 

机构地区：[1]黑龙江八一农垦大学食品学院,黑龙江大庆163319 [2]国家杂粮工程技术研究中心,黑龙江大庆163319

出　　处：《东北农业科学》2016年第3期8-13,共6页Journal of Northeast Agricultural Sciences

基　　金：星火计划项目(2015GA670008);国家科技支撑计划(2012BAN304);黑龙江省农垦总局技术开发项目(HNK13KF-01-01);大庆市科技局创新项目(sjh-2013-65)

摘　　要：[目的]探究黑龙江省、吉林省谷子种质资源的遗传基础和遗传多样性。[方法]利用黑、吉两省20个典型谷子品种及5对扩增良好且具有多态性的谷子SSR引物,分析各引物等位基因数、位点杂合度、Shannon′s指数（I）、有效等位基因数（Ne）及两省谷子品种聚类分析结果。[结果]每对引物检测出的等位基因数为4~6个,共得到26个等位基因,平均5.2个;各引物的多态信息含量（PIC）在0.645~0.767间变化,其中引物DG2831最高,引物QG7172最低。不同引物间位点杂合度、Shannon′s指数（I）、有效等位基因数（Ne）均存在差异,DG2831、JG13656分别是黑、吉两省的位点杂合度及多态信息含量最高的引物。对20个谷子品种进行聚类分析,在遗传相似系数在0.64处,20个谷子品种可聚为3大类。[结论]吉林省谷子品种基因多样性略比黑龙江省丰富;部分同地区的谷子品种聚为一类,说明不同地理来源的谷子资源,一部分品种的亲缘关系可能与地理来源有关。Objective of the study was to understand the genetic diversity of millet germplasm resources in Heilongjiang Province and Jilin Province. Alleles, loci heterozygosity, Shannon＇s index and effective number of genes （Ne） of 20 typical foxtail millets from Heilongjiang and Jilin provinces were analyzed by 5 pairs of SSR primers. Results showed that the number of alleles per primer was 4 -6, 26 alleles were obtained, and the average was 5.2. The polymorphism information content （PIC） of each primer was ranging from 0.645 to 0.767, with the maximum primer EG75638 and the lowest primer QG7172. Loci heterozygosity, Shannon＇s index （I） and effective number of genes （Ne） were different in different primers. The highest loci heterozygosity and polymorphism information content of each province were derived from different primers, which were JG13656 and DG2831. Cluster analysis of 20 variet- ies of foxtail millet in the genetic similarity coefficient 0.64 showed these varieties could be grouped into 3 categories. The primer analysis of two provinces showed that the genetic diversity of Jilin Province was higher than that of Heilongjiang Province. Parts of varieties from same geographical got together, it means that genetic relationship was related to geographic origin.

关 键 词：谷子 SSR 遗传多样性 遗传相似系数 聚类分析 

分 类 号：S515[农业科学—作物学]