抗玉米灰斑病相关基因的DDRT-PCR分析  

Analysis of Corn Gray Leaf Spot Resistance Related Genes with DDRT-PCR

作　　者：贾娇[1] 马莹莹[1] 苏前富[1] 孟灵敏[1] 张伟[1] 李红[1] 晋齐鸣[1] 

机构地区：[1]吉林省农业科学院/农业部东北作物有害生物综合治理重点实验室,吉林公主岭136100

出　　处：《东北农业科学》2016年第3期46-50,共5页Journal of Northeast Agricultural Sciences

基　　金：国家玉米产业技术体系(CARS-02)

摘　　要：为深入了解玉米抗灰斑病的分子机理,本试验以抗/感玉米灰斑病自交系78599-1、OH43Ht N/掖478、K12为试验材料,利用DDRT-PCR方法筛选玉米6叶期时接种玉米灰斑病菌粗毒素0、5、7和9 d时玉米叶片中差异表达的m RNA并通过反式Northern进行验证。结果发现在抗/感材料78599-1、OH43Ht N/掖478、K12中分别分离到6、5、5和3条差异表达片段;对19条差异表达片段进行测序和blast分析,得到6个候选基因片段,它们与玉米中MTN3、CONSTANS-CO5家族的基因、脱落酸受体基因和酰基辅酶A结合蛋白基因同源性分别为97%、99%、97%和100%;另外获得2个基因片段与未知功能基因同源性均为99%。研究结果将为深入研究抗灰斑病分子机理提供理论依据。In order to investigate the resistant molecular mechanism of corns against gray leaf spot, resistant inbred 78599-1, OH43HtN and susceptible inbred Ye478, K12 were used to screen differential expression gene through DDRT-PCR when 6 leaf stages inoculated. The differential expression genes were tested by RT-Northern and the positive clones were sequenced. Results showed that there were 6,5,5 and 3 positive genes in 78599-1, OH43HtN and Ye478, K12, respectively. The 6 differential expression genes were selected as candidate genes through the blast analysis of the 19 differential expression genes. The homology research indicated that candidate genes were highly homological to Zea mays MTN3, CONSTANS-C05, abscisic acid receptor （PYL） and CoA with 97%, 99%, 97% and 100% identity, respectively. The two unknown candidate genes were highly homological to Zea mays hypothetical protein with 99% identity. This study would provide some useful information for molecular breeding resistant to gray leaf spot.

关 键 词：玉米灰斑病 抗性基因 DDRT-PCR 抗/感自交系 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]