水稻响应稻瘟病菌胁迫的cDNA-AFLP分析  

Analysis of the cDNA-AFLP on Rice Response to Magnaporthe grisea Stress

作　　者：张俊华[1] 常浩[1] 陈宇飞[2] 潘春清[1] 刘扬[1] 牟明[1] 李云鹏[1] 

机构地区：[1]东北农业大学农学院,哈尔滨150030 [2]东北农业大学应用技术学院,哈尔滨150030

出　　处：《东北农业科学》2016年第4期70-74,共5页Journal of Northeast Agricultural Sciences

基　　金：哈尔滨市应用技术研究与开发项目(2014AB6BN036);黑龙江省粮食作物新品种选育及持续增产栽培技术创新平台项目(2011计划项目);黑龙江省教育厅科学技术研究项目(11551041);博士后研究人员落户黑龙江科研启动资助项目(LBH-Q09172)

摘　　要：本研究采用cDNA-AFLP技术建立了水稻与稻瘟病菌互作的基因表达图谱,对差异表达基因功能进行分析。利用64对引物进行筛选,共获得了960个转录衍生片段(transcript-derived fragment TDF)。回收测序200条片段,获得134条可读序列,通过BLAST比对分析,得到74个不重复的差异TDFs。对74个TDFs对应的聚丙烯酰胺凝胶上表达变化情况进行统计,接种稻瘟病菌后基因诱导表达上调多余下调表达,早期上调表达基因有6个,晚期上调表达基因有11个,其他形式上调表达基因32个,各种下调表达基因25个。In this study, the cDNA-AFLP technique was used to establish the gene expression profiles of rice infect-ed by Magnaporthe grisea, and the function of differential display expressed genes was analyzed. Nine hundred sixtytranscript derived fragments were obtained by using 64 pairs of primers. Two hundred fragments were recovered andsequenced, one hundred thirty four readable sequences were obtained. Seventy four non duplicated TDFs were got-ten by BLAST analysis. Among the statistical expression of 74 TDFs corresponding on PAGE, the number of up-reg-ulation genes was more than down-regulation genes after innoculated by Magnaporthe grisea, six were early up-reg-ulation genes, eleven were late up-regulation genes, thirty two were other forms of up-regulation genes, the totalnumber of down-regulation genes was 25.

关 键 词：水稻 稻瘟病菌 胁迫表达 CDNA-AFLP TDF 

分 类 号：S435.111.41[农业科学—农业昆虫与害虫防治]