槐米中黄酮类成分的微乳薄层色谱分离鉴定  

Isolation and identification of flavonoids in flos sophorae immaturus by microemulsion thin-layer chromatography

作　　者：赵惠茹[1] 郭德喜 李娜[3] 靖会[1] 杨静[1] 杨阳[1] 侯进[4] 

机构地区：[1]西安医学院药学院,陕西西安710021 [2]宁夏医科大学药学院,宁夏银川750004 [3]陕西师范大学西北濒危药材资源开发国家工程实验室,陕西西安710119 [4]西安医学院基础医学部,陕西西安710021

出　　处：《化工科技》2016年第4期27-30,40,共5页Science & Technology in Chemical Industry

基　　金：陕西省教育厅科研计划项目(15JK1638);陕西省科技厅自然科学基金(2015JM8449)

摘　　要：以聚酰胺为固定相,十二烷基硫酸钠-正丁醇-正己烷-水组成的微乳体系为展开剂,考察微乳液类型、表面活性剂、助表面活性剂、油相、改性剂对槐米中黄酮类成分的微乳薄层色谱分离效果的影响。结果表明,与V(甲醇)∶V(水)=1∶1和V(乙酸乙酯)∶V(甲酸)∶V(水)=8∶1∶1等常用展开剂相比,以φ(水)=75%的微乳液-甲酸(体积比9∶1)为展开剂时,分离得到的斑点数目多,斑点圆整不拖尾、荧光强度强,对槐米中的黄酮类成分的检测效果和灵敏度显著提高,可用于槐米中黄酮类成分的分离鉴定。With the flavonoids as target component,the samples were eluted by the developer of SDS/ n-C4HgOH/n-C6H14/H20.The influence of the type of microemulsion, surfactant, co-surfactant, oil phase and modifier on flavonoids in flos sophorae immaturus Was investigated through polyacetamide thin-layer chromatography.The studies showed that the optimum developer of microemulsion contains 75% water-formic acid （9：1）.Compared with commonly used expansion agent such as ethyl acetate- formic acid-water （8：1 ：1） and methanol-water （1 ： 1） ,there were more isolated rounded spots and fluorescence intensity. The separation effect by microemulsion thin-layer chromatography was improved significantly,the method can be used for the qualitative identification of flavonoids in flos so- phorae immaturus.

关 键 词：槐米 黄酮 微乳 薄层色谱 

分 类 号：R284[医药卫生—中药学]