川芎嗪对大鼠肾缺血再灌注损伤的保护作用及其机制  

Effect of ligustrazine on acute renal ischemia reperfusion injury of rats and investigate its mechanisms of protective effect on renal injury

作　　者：王娜 许海江[2] 赵春玲[2] 

机构地区：[1]杞县人民医院中药房,河南杞县475200 [2]郑州大学第一附属医院药剂科,河南郑州450002

出　　处：《山西中医学院学报》2016年第4期19-21,共3页Journal of Shanxi College of Traditional Chinese Medicine

摘　　要：目的:观察川芎嗪防治急性肾缺血再灌注损伤(RIRI)的作用,探讨其损伤保护作用的可能机制。方法:选择SD大鼠50只,随机分成假手术组、对照组、川芎嗪低、中、高剂量组,每组各10只。各组分别于术前和术后24 h取血标本,检测血清肌酐(SCr)、尿素氮(BUN)含量;各组于术后24 h再次麻醉,摘取左肾,作组织学分析及检测组织匀浆中SOD、MDA和TNF-α含量。结果:与对照组比较,川芎嗪低、中、高剂量组肾组织学评分、血清SCr、BUN含量以及肾组织MDA和TNF-α水平明显降低,SOD活性升高,并呈剂量依赖关系。结论:川芎嗪对急性肾缺血再灌注损伤具有保护作用,提高肾组织SOD活性、降低MDA和TNF-α水平是其减轻损伤的可能机制。Objective：To observe the effect of ligustrazine on acute renal ischemia reperfusion injury of rats and investigate its mechanisms of protective effect on renal injury. Methods：50 SD rats were randomly divided into sham operation group, control group and ligustrazine group of low,medium and high dosage. Blood samples were taken before operation and 24 h after operation respectively,and contents of serum creatinine （SCr）,urea nitrogen （BUN）were detected. All the rats were anaesthetized 24 h after operation again,and left kidney was removed,then the contents of SOD,MDA and TNF-α in tissue homogenate were detected and histological analysis was performed. Results：Compared with control group,contents of SCr,BUN,MDA and TNF-α and renal histology score were decreased and SOD activity was increased in ligustrazine groups of low,medium and high dosage with positive dose-dependent relationship. Conclusion：Ligustrazine has protective effect on acute renal ischemia reperfusion injury of rats through improving SOD activity of renal tissue and decreasing its contents of MDA and TNF-α.

关 键 词：肾脏 再灌注损伤 川芎嗪 超氧化物歧化酶 丙二醛 肿瘤坏死因子 

分 类 号：R285.5[医药卫生—中药学]