气相色谱法测定冬瓜子中4种脂肪酸的含量  

Determination of content of four kinds of fatty acids in Benincasa hispida seed by GC

作　　者：李慧峰[1] 荆然[1] 程艳刚 裴妙荣[1] 

机构地区：[1]山西中医学院,山西太原030024 [2]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《山西中医学院学报》2016年第4期22-24,共3页Journal of Shanxi College of Traditional Chinese Medicine

基　　金：基金项目 山西省中药材;中药饮片地方标准研究(2012009A)

摘　　要：目的：建立测定不同产地冬瓜子中4种脂肪酸含量的气相色谱方法。方法：色谱柱为HP-FFAP（0.2 mm×50 m）毛细管色谱柱，载气为氮气，流速0.5 mg/mL，分流比为23∶1，空气流速450 mg/mL，氢气流速45 mg/mL。柱温采取程序升温，进样口温度为250℃，FID检测器温度为250℃。结果：冬瓜子中棕榈酸质量分数为2.429%~2.977%，硬脂酸质量分数为1.217%~1.615%，油酸质量分数为3.260%~4.287%，亚油酸质量分数为10.752%~14.562%。结论：该方法准确度高、重复性好，可用于冬瓜子脂肪油中4种脂肪酸的质量控制。Objective：To establish a method for the determination of the content of four kinds fatty acids in benincasa hispida （Thunb.）Cogn.from different origins. Method：The GC was performed on HP-FFAP column （0.22 mm×50 m,0.3μm）,nitrogen gas was used as carrier and flow rate was 0.5 mL/min,split ratio was 23∶1,the air flow rate was 450 mL/min,the hydrogen flow rate was 45 mL/min. The column temperature was taken programmed,and the temperature of injector and FID detector was both at 250 ℃. Result：The content of palmtinic acid,octadecanoic acid,oleic acid and linoleic acid in benincasa hispida seed were 2.325%~2.985%,1.198% ~1.771%,2.212%~5.159%,7.383%~13.578%respectively. Conclusion：The method can be used to control the quality of four kinds fatty acids in benincasa hispida seed,which is accurate,convenient and repeatable.

关 键 词：气相色谱法 冬瓜子 油酸 亚油酸 棕榈酸 硬脂酸 

分 类 号：R285[医药卫生—中药学]