乙型肝炎肝硬化患者HBV复制对外周血单个核细胞FoxP^3 mRNA基因表达的影响  

Effects of HBV replication on the expression of FoxP^3 mRNA of peripheral blood mononuclear cells in patients with cirrhosis

作　　者：李芹 邓存良 张建军 李娜 

机构地区：[1]西南医科大学附属医院感染科,四川泸州646000

出　　处：《泸州医学院学报》2016年第4期366-368,共3页Journal of Luzhou Medical College

摘　　要：目的:探讨乙型肝炎肝硬化患者HBV复制对外周血单个核细胞叉头状螺旋转录因子P^3(forkhead winged helix transcription factor P^3,Fox P^3)m RNA基因表达的影响。方法 :提取正常组、慢性乙肝组、肝硬化组血液标本中的RNA,采用realtime PCR扩增法检测3组病例Fox P^3 m RNA基因表达,实时荧光定量检测HBV-DNA。结果 :慢性乙肝组、肝硬化组Fox P^3m RNA基因表达水平高于对照组,差异具有统计学意义(P<0.05);HBV-DNA与Fox P^3m RNA呈正相关(r=0.830,P=0.031)。结论 :在慢性乙肝、肝硬化组,Fox P^3 m RNA较正常对照组明显升高,提示慢性肝病时机体免疫受到抑制,导致HBVDNA复制活跃,HBV持续感染,乙肝慢性化。Objective： To investigate the influence of HBV replication on the expression of Fox P3 m RNA of peripheral blood mononuclear cells in patients with cirrhosis. Method： The total RNA was isolated from peripheral blood of the healthy control group, CHB group and Cirrhosis group. Then the Fox P3 m RNA expression was evaluated with real-time PCR. The HBV-DNA loads were measured with real-time fluorogenetic quantitative PCR. Results： In CHB and Cirrhosis groups, Fox P3 m RNA expression levels were significantly higher than that in the control group; and there is a positive correlation between HBV-DNA and Fox P3 m RNA（r = 0.830, P =0.031）. Conclusion： Fox P3 m RNA expression levels were significantly higher in both CHB and Cirrhosis groups than in control group, suggesting the depressed immune reaction, and increased HBVDNA replication, and thus leading to the persistence of HBV infection and chronic hepatitis.

关 键 词：叉头状螺旋转录因子p3 慢性乙型肝炎 肝硬化 基因表达 

分 类 号：R512.62[医药卫生—内科学] Q344.13[医药卫生—临床医学]